Cadmium accumulation is enhanced by ammonium compared to nitrate in two hyperaccumulators, without affecting speciation

Cheng, Miaomiao, Wang, Peng, Kopittke, Peter M., Wang, Anan, Sale, Peter W. G. and Tang, Caixian (2016) Cadmium accumulation is enhanced by ammonium compared to nitrate in two hyperaccumulators, without affecting speciation. Journal of Experimental Botany, 67 17: 5041-5050. doi:10.1093/jxb/erw270


Author Cheng, Miaomiao
Wang, Peng
Kopittke, Peter M.
Wang, Anan
Sale, Peter W. G.
Tang, Caixian
Title Cadmium accumulation is enhanced by ammonium compared to nitrate in two hyperaccumulators, without affecting speciation
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 1460-2431
0022-0957
Publication date 2016-09-01
Year available 2016
Sub-type Article (original research)
DOI 10.1093/jxb/erw270
Open Access Status DOI
Volume 67
Issue 17
Start page 5041
End page 5050
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Subject 1314 Physiology
1110 Plant Science
Abstract Nitrogen fertilization could improve the efficiency of Cd phytoextraction in contaminated soil and thus shorten the remediation time. However, limited information is available on the effect of N form on Cd phytoextraction and associated mechanisms in plants. This study examined the effect of N form on Cd accumulation, translocation, and speciation in Carpobrotus rossii and Solanum nigrum. Plants were grown in nutrient solution with 5-15 μM Cd in the presence of 1000 μM NH or NO . Plant growth and Cd uptake were measured, and Cd speciation was analyzed using synchrotron-based X-ray absorption spectroscopy. Shoot Cd accumulation was 30% greater with NH than NO supply. Carpobrotus rossii accumulated three times more Cd than S. nigrum. However, Cd speciation in the plants was not influenced by N form, but it did vary with species and tissues. In C. rossii, up to 91% of Cd was bound to S-containing ligands in all tissues except the xylem sap where 87-95% were Cd-OH complexes. Furthermore, the proportion of Cd-S in shoots was substantially lower in S. nigrum (44-69%) than in C. rossii (60-91%). It is concluded that the application of NH (instead of NO ) increased shoot Cd accumulation by increasing uptake and translocation, rather than changing Cd speciation, and is potentially an effective approach for increasing Cd phytoextraction.
Formatted abstract
Nitrogen fertilization could improve the efficiency of Cd phytoextraction in contaminated soil and thus shorten the remediation time. However, limited information is available on the effect of N form on Cd phytoextraction and associated mechanisms in plants. This study examined the effect of N form on Cd accumulation, translocation, and speciation in Carpobrotus rossii and Solanum nigrum. Plants were grown in nutrient solution with 5-15 μM Cd in the presence of 1000 μM NH4+ or NO3-. Plant growth and Cd uptake were measured, and Cd speciation was analyzed using synchrotron-based X-ray absorption spectroscopy. Shoot Cd accumulation was 30% greater with NH4+ than NO3- supply. Carpobrotus rossii accumulated three times more Cd than S. nigrum. However, Cd speciation in the plants was not influenced by N form, but it did vary with species and tissues. In C. rossii, up to 91% of Cd was bound to S-containing ligands in all tissues except the xylem sap where 87-95% were Cd-OH complexes. Furthermore, the proportion of Cd-S in shoots was substantially lower in S. nigrum (44-69%) than in C. rossii (60-91%). It is concluded that the application of NH4+ (instead of NO3-) increased shoot Cd accumulation by increasing uptake and translocation, rather than changing Cd speciation, and is potentially an effective approach for increasing Cd phytoextraction.
Keyword Carpobrotus rossii
Cd speciation
Cd translocation
Halophytes
Nitrogen form
Phytoremediation
Solanum nigrum
Synchrotron
XANES
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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