Heritability of targeted gene modifications induced by plant-optimized CRISPR systems

Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2016) Heritability of targeted gene modifications induced by plant-optimized CRISPR systems. Cellular and Molecular Life Sciences, 74 6: 1-19. doi:10.1007/s00018-016-2380-1


Author Mao, Yanfei
Botella, Jose Ramon
Zhu, Jian-Kang
Title Heritability of targeted gene modifications induced by plant-optimized CRISPR systems
Journal name Cellular and Molecular Life Sciences   Check publisher's open access policy
ISSN 1420-9071
1420-682X
Publication date 2016-09-27
Year available 2017
Sub-type Article (original research)
DOI 10.1007/s00018-016-2380-1
Open Access Status Not yet assessed
Volume 74
Issue 6
Start page 1
End page 19
Total pages 19
Place of publication Basel, Switzerland
Publisher Springer Basel AG
Language eng
Subject 1313 Molecular Medicine
1312 Molecular Biology
3004 Pharmacology
2804 Cellular and Molecular Neuroscience
1307 Cell Biology
Abstract The Streptococcus-derived CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR-associated protein 9) system has emerged as a very powerful tool for targeted gene modifications in many living organisms including plants. Since the first application of this system for plant gene modification in 2013, this RNA-guided DNA endonuclease system has been extensively engineered to meet the requirements of functional genomics and crop trait improvement in a number of plant species. Given its short history, the emphasis of many studies has been the optimization of the technology to improve its reliability and efficiency to generate heritable gene modifications in plants. Here we review and analyze the features of customized CRISPR/Cas9 systems developed for plant genetic studies and crop breeding. We focus on two essential aspects: the heritability of gene modifications induced by CRISPR/Cas9 and the factors affecting its efficiency, and we provide strategies for future design of systems with improved activity and heritability in plants.
Formatted abstract
The Streptococcus-derived CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR-associated protein 9) system has emerged as a very powerful tool for targeted gene modifications in many living organisms including plants. Since the first application of this system for plant gene modification in 2013, this RNA-guided DNA endonuclease system has been extensively engineered to meet the requirements of functional genomics and crop trait improvement in a number of plant species. Given its short history, the emphasis of many studies has been the optimization of the technology to improve its reliability and efficiency to generate heritable gene modifications in plants. Here we review and analyze the features of customized CRISPR/Cas9 systems developed for plant genetic studies and crop breeding. We focus on two essential aspects: the heritability of gene modifications induced by CRISPR/Cas9 and the factors affecting its efficiency, and we provide strategies for future design of systems with improved activity and heritability in plants.
Keyword CRISPR-Cas
Gene editing
Inheritance
Mutagenesis
SgRNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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