Angiotensin- I- converting enzyme (ACE) inhibitory peptides from Pacific cod skin gelatin using ultrafiltration membranes

Ngo, Dai-Hung, Vo, Thanh-Sang, Ryu, BoMi and Kim, Se-Kwon (2016) Angiotensin- I- converting enzyme (ACE) inhibitory peptides from Pacific cod skin gelatin using ultrafiltration membranes. Process Biochemistry, 51 10: 1622-1628. doi:10.1016/j.procbio.2016.07.006

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Author Ngo, Dai-Hung
Vo, Thanh-Sang
Ryu, BoMi
Kim, Se-Kwon
Title Angiotensin- I- converting enzyme (ACE) inhibitory peptides from Pacific cod skin gelatin using ultrafiltration membranes
Journal name Process Biochemistry   Check publisher's open access policy
ISSN 1359-5113
1873-3298
Publication date 2016-10-01
Sub-type Article (original research)
DOI 10.1016/j.procbio.2016.07.006
Open Access Status File (Author Post-print)
Volume 51
Issue 10
Start page 1622
End page 1628
Total pages 7
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Angiotensin- I- converting enzyme (ACE) is crucial in the control of hypertension and the development of type- 2 diabetes and other diseases associated with metabolic syndrome. The aim of this work was to utilize Pacific cod skin to purify ACE inhibitory peptides. First, gelatin was extracted from Pacific cod skin and hydrolyzed with several enzymes (pepsin, papain, α-chymotrypsin, trypsin, neutrase, and alcalase). The pepsin hydrolysate showed the strongest ACE inhibitory effect and was further fractionated into different ranges of molecular weight (<1, 1–5, 5–10, and >10 kDa) using ultrafiltration (UF) membranes. The peptic hydrolysate below 1 kDa resulted in two potent ACE inhibitory peptides, GASSGMPG (662 Da) and LAYA (436 Da), with IC50 values (concentration required to decrease the ACE activity by 50%) of 6.9 and 14.5 μM, respectively. Moreover, to explore the interaction between the peptides and ACE molecule, the tertiary structure of ACE and docking simulation to the peptides were predicted using Docking Server. Pacific cod peptides can be used as functional food ingredients to prevent hypertension and its related diseases.
Keyword Antihypertension
Bioactive peptides
Functional ingredients
Molecular docking
Pacific cod skin
Ultrafiltration membrane
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Pharmacy Publications
 
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