A genetic diversity study of antifungal Lactobacillus plantarum isolates

Dong, An-Ran, Thuy Ho, Van Thi, Lo, Raquel, Bansal, Nidhi and Turner, Mark S. (2017) A genetic diversity study of antifungal Lactobacillus plantarum isolates. Food Control, 72 A: 83-89. doi:10.1016/j.foodcont.2016.07.026

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Author Dong, An-Ran
Thuy Ho, Van Thi
Lo, Raquel
Bansal, Nidhi
Turner, Mark S.
Title A genetic diversity study of antifungal Lactobacillus plantarum isolates
Formatted title
A genetic diversity study of antifungal Lactobacillus plantarum isolates
Journal name Food Control   Check publisher's open access policy
ISSN 0956-7135
1873-7129
Publication date 2017-02-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.foodcont.2016.07.026
Open Access Status File (Author Post-print)
Volume 72
Issue A
Start page 83
End page 89
Total pages 7
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Formatted abstract
Lactobacillus plantarum is a lactic acid bacterium commonly found on fruits and vegetables and also used in a variety of food fermentations. Strains from this species are also regularly reported as having antifungal or probiotic activity. Genotyping methods can be used to differentiate strains of the same species thus determining if strains are related or not. However for L. plantarum, the currently used methods have limitations including DNA band profile interpretation difficulty and cost. In this study, a new genotyping method based on multi-locus variable number tandem repeat analysis (MLVA) was developed and compared to a previously reported randomly amplified polymorphic DNA-PCR (RAPD-PCR) method for L. plantarum. With a selection of 13 antifungal strains of L. plantarum isolated from heterogeneous sources (cheese, silage, sauerkraut, vegetables and a probiotic product), RAPD-PCR revealed 9 different profiles resulting in a Hunter-Gaston discrimination index (D-value) of 0.94. The new MLVA method which compares the lengths of 4 repetitive regions within LPXTG motif-containing surface protein genes differentiated the 13 L. plantarum strains into 10 different subtypes leading to a D-value of 0.95. Interestingly 11 additional L. plantarum isolates obtained in a previous study during a screen for antifungal activity against the common cheese spoilage mould Penicillium commune all possessed the same RAPD-PCR and MLVA profile as each other and the commercial probiotic strain L. plantarum 299v. This study demonstrates that the new MLVA method can be used to simply and inexpensively differentiate L. plantarum strains and provide information regarding strain relatedness and thus potential insight into strain properties.
Keyword Antifungal
Lactobacillus plantarum
MLVA
Penicillium commune
Typing
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
 
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