The down-regulation of the mitogenic fibrinogen receptor (MFR) in serum-containing medium does not occur in defined medium

Levesque, JP, Hatzfeld, A, Domart, I and Hatzfeld, J (1990) The down-regulation of the mitogenic fibrinogen receptor (MFR) in serum-containing medium does not occur in defined medium. Experimental Cell Research, 186 2: 257-263. doi:10.1016/0014-4827(90)90304-S


Author Levesque, JP
Hatzfeld, A
Domart, I
Hatzfeld, J
Title The down-regulation of the mitogenic fibrinogen receptor (MFR) in serum-containing medium does not occur in defined medium
Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 0014-4827
Publication date 1990-01-01
Sub-type Article (original research)
DOI 10.1016/0014-4827(90)90304-S
Volume 186
Issue 2
Start page 257
End page 263
Total pages 7
Language eng
Subject 1307 Cell Biology
Abstract Normal human hemopoietic cells such as early bone marrow progenitors, or lymphoma-derived cell lines such as Raji or JM cells, possess a low-affinity receptor specific for fibrinogen. This receptor triggers a mitogenic effect. It differs from the glycoprotein IIb-IIIa which is involved in fibrinogen-induced platelet aggregation. We demonstrate here that this mitogenic fibrinogen receptor (MFR) can be internalized or reexpressed, depending on culture conditions. Internalization was temperature-dependent. At 37 °C in the presence of cycloheximide or actinomycin D, the half-life of cell surface MFRs was 2 h, independent of receptor occupancy. Binding of fibrinogen to the MFR resulted in a down-regulation which was fibrinogen dose-dependent. This occurred in serum-supplemented medium but not in defined medium supplemented with fatty acids. Reexpression of MFRs could be induced in 28 to 42 h by serum removal. The down-regulation of mitogenic receptors in plasma or serum could explain why normal cells do not proliferate in the peripheral blood.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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