Effects of binary mixtures of benzo[a]pyrene, arsenic, cadmium, and lead on oxidative stress and toxicity in HepG2 cells

Muthusamy, Sasikumar, Peng, Cheng and Ng, Jack (2016) Effects of binary mixtures of benzo[a]pyrene, arsenic, cadmium, and lead on oxidative stress and toxicity in HepG2 cells. Chemosphere, 165 41-51. doi:10.1016/j.chemosphere.2016.08.137


Author Muthusamy, Sasikumar
Peng, Cheng
Ng, Jack
Title Effects of binary mixtures of benzo[a]pyrene, arsenic, cadmium, and lead on oxidative stress and toxicity in HepG2 cells
Journal name Chemosphere   Check publisher's open access policy
ISSN 0045-6535
1879-1298
Publication date 2016-12-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.chemosphere.2016.08.137
Open Access Status Not yet assessed
Volume 165
Start page 41
End page 51
Total pages 11
Place of publication Kidlington, Oxford United Kingdom
Publisher Pergamon Press
Language eng
Abstract Mixed contamination of benzo[a]pyrene (B[a]P), arsenic (As), cadmium (Cd), and lead (Pb) is a major environmental and human health concern. The mixture toxicity data on these co-contaminants are important for their risk assessment. In this study, we have determined the mixture toxicity of As, Cd and Pb, and B[a]P with As, Cd or Pb in HepG2 cells. The binary mixtures of Cd + As, Cd + Pb and As + Pb and B[a]P + metals (B[a]P + As, B[a]P + Cd and B[a]P + Pb) were evaluated for their interaction on the cytotoxicity using the MTS assay. A full factorial design (4 × 5) was used to determine the interaction toxicity and all the six mixtures showed significant interaction on the cytotoxicity. We further investigated the role of oxidative stress (reactive oxygen species (ROS) generation) and antioxidant defense mechanism (total glutathione (GSH) level) with the observed cytotoxicity. The mixtures of metals reduced the total GSH level and increased the ROS generation, respectively. In the case of mixtures of B[a]P and metals, both total GSH level and ROS generation were increased. Overall, the binary mixtures of metals and B[a]P with metals caused a dose dependent toxicity to HepG2 cells. The results also showed a significant contribution of oxidative stress to the observed toxicity and the potential protective role of the total GSH level against this mixture toxicity. The findings of interaction between B[a]P and metals might have an impact on the potential human health risk of this mixtures at contaminated sites.
Keyword Binary mixtures
Cytotoxicity
Oxidative stress
Interaction toxicity
Risk assessment
In vitro assays
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
National Research Centre for Environmental Toxicology Publications
 
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Created: Tue, 04 Oct 2016, 19:35:02 EST by Sasikumar Muthusamy on behalf of National Res Centre For Environmental Toxicology