Increased cocaine-induced conditioned place preference during periadolescence in maternally separated male BALB/c mice: the role of cortical BDNF, microRNA-212, and MeCP2

Viola, Thiago Wendt, Wearick-Silva, Luis Eduardo, De Azeredo, Lucas Araujo, Centeno-Silva, Anderson, Murphy, Conor, Marshall, Paul, Li, Xiang, Singewald, Nicolas, Garcia, Frederico, Bredy, Timothy W. and Grassi-Oliveira, Rodrigo (2016) Increased cocaine-induced conditioned place preference during periadolescence in maternally separated male BALB/c mice: the role of cortical BDNF, microRNA-212, and MeCP2. Psychopharmacology, 233 17: 3279-3288. doi:10.1007/s00213-016-4373-z


Author Viola, Thiago Wendt
Wearick-Silva, Luis Eduardo
De Azeredo, Lucas Araujo
Centeno-Silva, Anderson
Murphy, Conor
Marshall, Paul
Li, Xiang
Singewald, Nicolas
Garcia, Frederico
Bredy, Timothy W.
Grassi-Oliveira, Rodrigo
Title Increased cocaine-induced conditioned place preference during periadolescence in maternally separated male BALB/c mice: the role of cortical BDNF, microRNA-212, and MeCP2
Journal name Psychopharmacology   Check publisher's open access policy
ISSN 1432-2072
0033-3158
Publication date 2016-09-01
Sub-type Article (original research)
DOI 10.1007/s00213-016-4373-z
Open Access Status Not yet assessed
Volume 233
Issue 17
Start page 3279
End page 3288
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2017
Language eng
Formatted abstract
Rationale: Early life stress is a major risk factor for cocaine addiction; however, the underlying molecular mechanisms remain relatively unexplored. MicroRNA-212 (miR-212) and methyl CpG binding protein 2 (MeCP2) have recently emerged as key regulators of brain-derived neurotrophic factor (BDNF) signaling during the acquisition and maintenance of cocaine-seeking behaviors.

Objectives: We therefore investigated the effect of maternal separation (MS) on cocaine-induced conditioned place preference (CPP) during periadolescence and how this influences miR-212, Mecp2, and Bdnf expressions in the prefrontal cortex.

Methods: Male BALB/c mice subjected to MS (3 h/day) from postnatal day 2 to 15 or normal animal facility rearing (AFR) were tested for CPP at postnatal day 45, or not exposed to experimental manipulations (drug-naïve animals). Cultured primary cortical neurons were used to determine miR-212 expression changes following depolarization by KCL treatment.

Results: MS increased cocaine-induced CPP and decreased Bdnf exon IV expression, which correlated with higher CPP scores in such animals. An experience-dependent decrease in miR-212 expression was observed following CPP test. This effect was mimicked in primary cortical neurons in vitro, under activity-dependent conditions. In contrast, increased Mecp2 expression was found after CPP test, suggesting an opposing relationship between miR-212 and Mecp2 expression following cocaine place preference acquisition. However, these effects were not present in mice exposed to MS.

Conclusions: Together, our results suggest that early life stress can enhance the motivational salience for cocaine-paired cues during periadolescence, and that altered expression of miR-212, Mecp2, and Bdnf in the prefrontal cortex is involved in this process.
Keyword Adolescence
Brain-derived neurotrophic factor
Cocaine
Conditioned place preference
Maternal separation
MicroRNAs
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 02 Oct 2016, 10:47:44 EST by System User on behalf of Learning and Research Services (UQ Library)