Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients

Nagel, Robyn, Traub, Rebecca J., Allcock, Richard J. N., Kwan, Marcella M. S. and Bielefeldt-Ohmann, Helle (2016) Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients. Microbiome, 4 1: 47. doi:10.1186/s40168-016-0191-0


Author Nagel, Robyn
Traub, Rebecca J.
Allcock, Richard J. N.
Kwan, Marcella M. S.
Bielefeldt-Ohmann, Helle
Title Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients
Formatted title
Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients
Journal name Microbiome   Check publisher's open access policy
ISSN 2049-2618
Publication date 2016-08-31
Year available 2016
Sub-type Article (original research)
DOI 10.1186/s40168-016-0191-0
Open Access Status DOI
Volume 4
Issue 1
Start page 47
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Abstract Background: We investigated whether the carriage of Blastocystis in IBS patients was associated with differences in the faecal microbiota. Forty patients with diarrhoea-predominant IBS (26 Blastocystis-positive and 14 Blastocystis-negative) and 57 healthy controls (HC) (42 Blastocystis-positive and 15 Blastocystis-negative) submitted faecal samples for metataxonomic analysis of the 16S ribosomal RNA gene. Differences in the relative abundance of bacteria in these IBS and HC groups were evaluated from phylum to genus level.
Formatted abstract
Background: We investigated whether the carriage of Blastocystis in IBS patients was associated with differences in the faecal microbiota. Forty patients with diarrhoea-predominant IBS (26 Blastocystis-positive and 14 Blastocystis-negative) and 57 healthy controls (HC) (42 Blastocystis-positive and 15 Blastocystis-negative) submitted faecal samples for metataxonomic analysis of the 16S ribosomal RNA gene. Differences in the relative abundance of bacteria in these IBS and HC groups were evaluated from phylum to genus level.

Results: Significant changes were observed in two dominant phyla in IBS patients, regardless of Blastocystis infection status, namely a rise in Firmicutes and a statistically significant reduction in relative abundance of Bacteroidetes (with a threefold increase in the Firmicutes to Bacteoridetes ratio). Significant differences at genus level in IBS subjects compared to HC were also observed for many bacterial species. However, further clinical subgroup analysis of Blastocystis-positive and Blastocystis-negative subjects, regardless of symptoms, showed no significant differences at the phylum or genus level in IBS-P compared to IBS-N.

Conclusions: Significant differences in the faecal microbiota between diarrhoea-predominant IBS patients and healthy controls were confirmed, but the carriage of Blastocystis did not significantly alter the faecal microbiota. If Blastocystis-positive patients represent a separate clinical subtype of IBS, this group is not identified by changes in the microbiota.
Keyword Faecal microbiota
Blastocystis
Irritable bowel syndrome
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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