Influenza nucleoprotein DNA vaccination by a skin targeted, dry coated, densely packed microprojection array (Nanopatch) induces potent antibody and CD8+ T cell responses

Fernando, Germain J. P., Zhang, Jin, Ng, Hwee-Ing, Haigh, Oscar L., Yukiko, Sally R. and Kendall, Mark A. F. (2016) Influenza nucleoprotein DNA vaccination by a skin targeted, dry coated, densely packed microprojection array (Nanopatch) induces potent antibody and CD8+ T cell responses. Journal of Controlled Release, 237 35-41. doi:10.1016/j.jconrel.2016.06.045

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Author Fernando, Germain J. P.
Zhang, Jin
Ng, Hwee-Ing
Haigh, Oscar L.
Yukiko, Sally R.
Kendall, Mark A. F.
Title Influenza nucleoprotein DNA vaccination by a skin targeted, dry coated, densely packed microprojection array (Nanopatch) induces potent antibody and CD8+ T cell responses
Journal name Journal of Controlled Release   Check publisher's open access policy
ISSN 1873-4995
0168-3659
Publication date 2016-09-10
Sub-type Article (original research)
DOI 10.1016/j.jconrel.2016.06.045
Open Access Status File (Author Post-print)
Volume 237
Start page 35
End page 41
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2017
Language eng
Formatted abstract
DNA vaccines have many advantages such as thermostability and the ease and rapidity of manufacture; for example, in an influenza pandemic situation where rapid production of vaccine is essential. However, immunogenicity of DNA vaccines was shown to be poor in humans unless large doses of DNA are used. If a highly efficacious DNA vaccine delivery system could be identified, then DNA vaccines have the potential to displace protein vaccines. In this study, we show in a C57BL/6 mouse model, that the Nanopatch, a microprojection array of high density (> 21,000 projections/cm2), could be used to deliver influenza nucleoprotein DNA vaccine to skin, to generate enhanced antigen specific antibody and CD8+ T cell responses compared to the conventional intramuscular (IM) delivery by the needle and syringe. Antigen specific antibody was measured using ELISA assays of mice vaccinated with a DNA plasmid containing the nucleoprotein gene of influenza type A/WSN/33 (H1N1). Antigen specific CD8+ T cell responses were measured ex-vivo in splenocytes of mice using IFN-γ ELISPOT assays. These results and our previous antibody and CD4+ T cell results using the Nanopatch delivered HSV DNA vaccine indicate that the Nanopatch is an effective delivery system of general utility that could potentially be used in humans to increase the potency of the DNA vaccines.
Keyword DNA vaccination
Enhanced immunogenicity
Microneedle
Needle-free vaccination
Skin vaccination
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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