Rapid extraction and identification of maitotoxin and ciguatoxin-like toxins from Caribbean and Pacific Gambierdiscus using a new functional bioassay

Lewis, Richard J., Inserra, Marco, Vetter, Irina, Holland, William C., Hardison, D. Ransom, Tester, Patricia A. and Litaker, R. Wayne (2016) Rapid extraction and identification of maitotoxin and ciguatoxin-like toxins from Caribbean and Pacific Gambierdiscus using a new functional bioassay. PLoS ONE, 11 7: . doi:10.1371/journal.pone.0160006


Author Lewis, Richard J.
Inserra, Marco
Vetter, Irina
Holland, William C.
Hardison, D. Ransom
Tester, Patricia A.
Litaker, R. Wayne
Title Rapid extraction and identification of maitotoxin and ciguatoxin-like toxins from Caribbean and Pacific Gambierdiscus using a new functional bioassay
Formatted title
Rapid extraction and identification of maitotoxin and ciguatoxin-like toxins from Caribbean and Pacific Gambierdiscus using a new functional bioassay
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2016-07-28
Sub-type Article (original research)
DOI 10.1371/journal.pone.0160006
Open Access Status DOI
Volume 11
Issue 7
Total pages 15
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Background: Ciguatera is a circumtropical disease produced by polyether sodium channel toxins (ciguatoxins) that enter the marine food chain and accumulate in otherwise edible fish. Ciguatoxins, as well as potent water-soluble polyethers known as maitotoxins, are produced by certain dinoflagellate species in the genus Gambierdiscus and Fukuyoa spp. in the Pacific but little is known of the potential of related Caribbean species to produce these toxins.

Methods: We established a simplified procedure for extracting polyether toxins from Gambierdiscus and Fukuyoa spp. based on the ciguatoxin rapid extraction method (CREM). Fractionated extracts from identified Pacific and Caribbean isolates were analysed using a functional bioassay that recorded intracellular calcium changes (Ca2+) in response to sample addition in SH-SY5Y cells. Maitotoxin directly elevated Ca2+i, while low levels of ciguatoxin-like toxins were detected using veratridine to enhance responses.

Results: We identified significant maitotoxin production in 11 of 12 isolates analysed, with 6 of 12 producing at least two forms of maitotoxin. In contrast, only 2 Caribbean isolates produced detectable levels of ciguatoxin-like activity despite a detection limit of >30 pM. Significant strain-dependent differences in the levels and types of ciguatoxins and maitotoxins produced by the same Gambierdiscus spp. were also identified.

Conclusions: The ability to rapidly identify polyether toxins produced by Gambierdiscus spp. in culture has the potential to distinguish ciguatoxin-producing species prior to large-scale culture and in naturally occurring blooms of Gambierdiscus and Fukuyoa spp. Our results have implications for the evaluation of ciguatera risk associated with Gambierdiscus and related species.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 02 Oct 2016, 10:19:42 EST by System User on behalf of Learning and Research Services (UQ Library)