MTMR4 is required for the stability of the salmonella-containing vacuole

Teo, Wei X., Kerr, Markus C. and Teasdale, Rohan D. (2016) MTMR4 is required for the stability of the salmonella-containing vacuole. Frontiers in Cellular and Infection Microbiology, 6 91: 1-11. doi:10.3389/fcimb.2016.00091

Author Teo, Wei X.
Kerr, Markus C.
Teasdale, Rohan D.
Title MTMR4 is required for the stability of the salmonella-containing vacuole
Formatted title
MTMR4 Is required for the stability of the salmonella-containing vacuole
Journal name Frontiers in Cellular and Infection Microbiology   Check publisher's open access policy
ISSN 2235-2988
Publication date 2016-08-30
Sub-type Article (original research)
DOI 10.3389/fcimb.2016.00091
Open Access Status DOI
Volume 6
Issue 91
Start page 1
End page 11
Total pages 11
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Language eng
Formatted abstract
The intracellular pathogen Salmonella enterica servovar Typhimurium (S.typhimurium) modulates the host cell's phosphoinositide (PI) metabolism to establish its intracellular replicative niche, the Salmonella-containing vacuole (SCV). Upon invasion, phosphoinositide 3-phosphate (PI(3)P) and other early endosomal markers are rapidly recruited to and remain associated with the SCV throughout its early maturation. While the phosphoinositide 3-phosphatase myotubularin 4 (MTMR4) has an established role in regulating autophagy and cellular PI(3)P-content, two processes associated with the intracellular survival of S. typhimurium, a direct role for MTMR4 in Salmonella biology has not been examined. Here we demonstrate that GFP-tagged MTMR4 is recruited to the SCV and infection of cells depleted of endogenous MTMR4 results in a decrease in viable intracellular Salmonella. This reflects a significant increase in the proportion of SCVs with compromised integrity, which targets the compartment for autophagy and consequent bacterial cell death. These findings highlight the importance of PI(3)P regulation to the integrity of the SCV and reveal a novel role for the myotubularins in bacterial pathogenesis.
Keyword Salmonella
Salmonella-containing vacuole
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article number 91

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
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Created: Sat, 01 Oct 2016, 00:10:46 EST by Susan Allen on behalf of Institute for Molecular Bioscience