Factors affecting the activation, motility and cryopreservation of the spermatozoa of the yellowfin bream, Acanthopagrus australis (Günther)

THOROGOOD J. and BLACKSHAW A. (1992) Factors affecting the activation, motility and cryopreservation of the spermatozoa of the yellowfin bream, Acanthopagrus australis (Günther). Aquaculture Research, 23 3: 337-344. doi:10.1111/j.1365-2109.1992.tb00776.x


Author THOROGOOD J.
BLACKSHAW A.
Title Factors affecting the activation, motility and cryopreservation of the spermatozoa of the yellowfin bream, Acanthopagrus australis (Günther)
Journal name Aquaculture Research   Check publisher's open access policy
ISSN 1365-2109
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2109.1992.tb00776.x
Open Access Status Not yet assessed
Volume 23
Issue 3
Start page 337
End page 344
Total pages 8
Subject 1104 Complementary and Alternative Medicine
Abstract Abstract. The effects of varying temperature, salinity and pH on the activation and subsequent motility of sperm of the yellowfin bream, Acanthopagrus australis (Günther), were assessed using a linear scale based on the overall activity of the sperm over time. Motility half‐life was calculated using log transformation. Conditions reflecting the natural habitat of the fish, oceanic salinity (1200 mOsM) and slight alkalinity (pH 8‐8‐5), were shown to produce both maximum activation and subsequent motility duration. The half‐life of activated sperm was shown to be greater at 4°C than at 20‐23°C (5·13 min:22·8 min). Storage of freshly stripped semen was shown to be most successful at 4°C with a half‐life of 98·9 min. The cryopreservation of semen was tested using the cryoprotectants glycerol and dimethyl sulphoxide at concentrations ranging from 0·5M to 2·0M, and pre‐freezing equilibration times of 5 and 15 min. Glycerol at 20 M was shown to give significantly superior results. There was no significant difference between sperm activation or sperm half‐life for fresh‐stripped semen and frozen semen, using glycerol at 2·0M as the cryoprotectant. Copyright
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 27 Sep 2016, 13:46:47 EST by System User