Triggering apoptosis in hematopoietic cells with cytotoxic drugs

Crowley, Lisa C., Marfell, Brooke J., Scott, Adrian P. and Waterhouse, Nigel J. (2016) Triggering apoptosis in hematopoietic cells with cytotoxic drugs. Cold Spring Harbor Protocols, 2016 7: 635-638. doi:10.1101/pdb.prot087130


Author Crowley, Lisa C.
Marfell, Brooke J.
Scott, Adrian P.
Waterhouse, Nigel J.
Title Triggering apoptosis in hematopoietic cells with cytotoxic drugs
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
1940-3402
Publication date 2016-07-01
Year available 2016
Sub-type Other
DOI 10.1101/pdb.prot087130
Open Access Status Not yet assessed
Volume 2016
Issue 7
Start page 635
End page 638
Total pages 4
Place of publication Cold Spring Harbor, NY United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Abstract Cytotoxic agents are commonly added to cultured cells in the laboratory to investigate their efficacy, mechanism of action, and therapeutic potential. Most of these agents trigger cell death by apoptosis, which is also the most common form of cell death during development, aging, homeostasis, and eradication of disease. Treatment of cells with cytotoxic agents is therefore useful for investigating basic mechanisms of cell death in the human body. Actinomycin D, a cytotoxic agent isolated from Streptomyces, induces apoptosis in a variety of cell lines including the histiocytic lymphoma cell line U937. Treatment of U937 cells with actinomycin D provides an ideal model of drug-induced apoptosis that can also be used as a positive control for comparison with other treatments.
Formatted abstract
Cytotoxic agents are commonly added to cultured cells in the laboratory to investigate their efficacy, mechanism of action, and therapeutic potential. Most of these agents trigger cell death by apoptosis, which is also the most common form of cell death during development, aging, homeostasis, and eradication of disease. Treatment of cells with cytotoxic agents is therefore useful for investigating basic mechanisms of cell death in the human body. Actinomycin D, a cytotoxic agent isolated from Streptomyces, induces apoptosis in a variety of cell lines including the histiocytic lymphoma cell line U937. Treatment of U937 cells with actinomycin D provides an ideal model of drug-induced apoptosis that can also be used as a positive control for comparison with other treatments.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Other
Collections: Mater Research Institute-UQ (MRI-UQ)
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Created: Thu, 22 Sep 2016, 20:34:52 EST by Kristen Gibbons on behalf of School of Medicine