Measuring cell death by trypan blue uptake and light microscopy

Crowley, Lisa C., Marfell, Brooke J., Christensen, Melinda E. and Waterhouse, Nigel J. (2016) Measuring cell death by trypan blue uptake and light microscopy. Cold Spring Harbor Protocols, 2016 7: 643-646. doi:10.1101/pdb.prot087155

Author Crowley, Lisa C.
Marfell, Brooke J.
Christensen, Melinda E.
Waterhouse, Nigel J.
Title Measuring cell death by trypan blue uptake and light microscopy
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
Publication date 2016-07-01
Sub-type Other
DOI 10.1101/pdb.prot087155
Open Access Status Not yet assessed
Volume 2016
Issue 7
Start page 643
End page 646
Total pages 4
Place of publication Cold Spring Harbor, NY United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
Abstract Trypan blue is a colorimetric dye that stains dead cells with a blue color easily observed using light microscopy at low resolution. The staining procedure is rapid and cells can be analyzed within minutes. The number of live (unstained) and dead (blue) cells can be counted using a hemocytometer on a basic upright microscope. Trypan blue staining is therefore a convenient assay for rapidly determining the overall viability of cells in a culture before commencing scientific experimentation, or for quantitating cell death following treatment with any cytotoxic stimuli.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Other
Collections: Mater Research Institute-UQ (MRI-UQ)
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Created: Thu, 22 Sep 2016, 20:34:22 EST by Kristen Gibbons on behalf of Learning and Research Services (UQ Library)