Measuring cell death by propidium iodide uptake and flow cytometry

Crowley, Lisa C., Scott, Adrian P., Marfell, Brooke J., Boughaba, Jeanne A., Chojnowski, Grace and Waterhouse, Nigel J. (2016) Measuring cell death by propidium iodide uptake and flow cytometry. Cold Spring Harbor Protocols, 2016 7: 647-651. doi:10.1101/pdb.prot087163


Author Crowley, Lisa C.
Scott, Adrian P.
Marfell, Brooke J.
Boughaba, Jeanne A.
Chojnowski, Grace
Waterhouse, Nigel J.
Title Measuring cell death by propidium iodide uptake and flow cytometry
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
1940-3402
Publication date 2016-07-01
Sub-type Other
DOI 10.1101/pdb.prot087163
Open Access Status Not yet assessed
Volume 2016
Issue 7
Start page 647
End page 651
Total pages 5
Place of publication Cold Spring Harbor, NY United States
Publisher Cold Spring Harbor Laboratory Press
Abstract Propidium iodide (PI) is a small fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. PI uptake versus exclusion can be used to discriminate dead cells, in which plasma membranes become permeable regardless of the mechanism of death, from live cells with intact membranes. PI is excited by wavelengths between 400 and 600 nm and emits light between 600 and 700 nm, and is therefore compatible with lasers and photodetectors commonly available in flow cytometers. This protocol for PI staining can be used to quantitate cell death in most modern research facilities and universities.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Other
Collections: Mater Research Institute-UQ (MRI-UQ)
HERDC Pre-Audit
School of Medicine Publications
 
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Created: Thu, 22 Sep 2016, 20:33:48 EST by Kristen Gibbons on behalf of Learning and Research Services (UQ Library)