Measuring survival of hematopoietic cancer cells with the Colony-forming assay in soft agar

Crowley, Lisa C. and Waterhouse, Nigel J. (2016) Measuring survival of hematopoietic cancer cells with the Colony-forming assay in soft agar. Cold Spring Harbor Protocols, 2016 8: 725-728. doi:10.1101/pdb.prot087189


Author Crowley, Lisa C.
Waterhouse, Nigel J.
Title Measuring survival of hematopoietic cancer cells with the Colony-forming assay in soft agar
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
1940-3402
Publication date 2016-08-01
Sub-type Other
DOI 10.1101/pdb.prot087189
Open Access Status Not yet assessed
Volume 2016
Issue 8
Start page 725
End page 728
Total pages 4
Place of publication NY United States
Publisher Cold Spring Harbor Laboratory Press
Abstract Colony-forming assays measure the ability of cells in culture to grow and divide into groups. Any cell that has the potential to form a colony may also have the potential to cause cancer or relapse in vivo. Colony-forming assays also provide an indirect measurement of cell death because any cell that is dead or dying will not continue to proliferate. The proliferative capacity of adherent cells such as fibroblasts can be determined by growing cells at low density on culture dishes and counting the number of distinct groups that form over time. Cells that grow in suspension, such as hematopoietic cells, cannot be assayed this way because the cells move freely in the media. Assays to determine the colony-forming ability of hematopoietic cells must therefore be performed in solid matrices that restrict large-scale movement of the cells. One such matrix is soft agar. This protocol describes the use of soft agar to compare the colony-forming ability of untreated hematopoietic cells to the colony-forming ability of hematopoietic cells that have been treated with a cytotoxic agent.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Other
Collections: Mater Research Institute-UQ (MRI-UQ)
HERDC Pre-Audit
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Thu, 22 Sep 2016, 20:29:28 EST by Kristen Gibbons on behalf of Learning and Research Services (UQ Library)