High-performance liquid chromatographic method for the quantitation of bupivacaine, 2,6-pipecoloxylidide and 4′-hydroxybupivacaine in plasma and urine

Kastrissios H., Hung M.-F. and Triggs E.J. (1992) High-performance liquid chromatographic method for the quantitation of bupivacaine, 2,6-pipecoloxylidide and 4′-hydroxybupivacaine in plasma and urine. Journal of Chromatography B: Biomedical Sciences and Applications, 577 1: 103-107. doi:10.1016/0378-4347(92)80603-N


Author Kastrissios H.
Hung M.-F.
Triggs E.J.
Title High-performance liquid chromatographic method for the quantitation of bupivacaine, 2,6-pipecoloxylidide and 4′-hydroxybupivacaine in plasma and urine
Journal name Journal of Chromatography B: Biomedical Sciences and Applications
ISSN 0378-4347
Publication date 1992-05-20
Sub-type Article (original research)
DOI 10.1016/0378-4347(92)80603-N
Volume 577
Issue 1
Start page 103
End page 107
Total pages 5
Subject 1600 Chemistry
1308 Clinical Biochemistry
1313 Molecular Medicine
Abstract A high-performance liquid chromatographic method with ultraviolet detection at 210 nm for quantitation of bupivacaine and two of its metabolites from plasma and urine is described. The compounds are extracted into n-hexane-isopropanol (5:1), evaporated and the reconstituted residue injected onto a reversed phase C18 column. Standard curves for all compounds were linear (r2 > 0.999) in the range 20-2000 ng/ml, with a limit of detection of 10 ng/ml. The inter-day coefficients of variation ranged between 2.7 and 12.2%. The method was applied to analyse bupivacaine and metabolite concentrations in patients on long-term epidural bupivacaine-fentanyl infusions.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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