Unculturable microbes detected by molecular sequences and probes

Liesack W. and Stackebrandt E. (1992) Unculturable microbes detected by molecular sequences and probes. Biodiversity and Conservation, 1 4: 250-262. doi:10.1007/BF00693763

Author Liesack W.
Stackebrandt E.
Title Unculturable microbes detected by molecular sequences and probes
Journal name Biodiversity and Conservation   Check publisher's open access policy
ISSN 0960-3115
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1007/BF00693763
Open Access Status Not yet assessed
Volume 1
Issue 4
Start page 250
End page 262
Total pages 13
Publisher Kluwer Academic Publishers
Subject 2309 Nature and Landscape Conservation
2303 Ecology
2300 Environmental Science
Abstract Nucleic acid probes have revolutionized rapid diagnostics of plant-, animal- and human-pathogenic viroids, viruses, bacteria and protozoa, and have complemented the spectrum of immunological and serological tests. Sequences of genes and rRNA, of proven potential for identification of taxa, are a valuable tool in that both the identity and the phylogenetic position of an isolate can be elucidated. On the other hand, this approach is still laborious and requires sophisticated equipment. Designation of probes does not require information about sequences, although such knowledge is extremely helpful in the formulation of highly specific oligonucleotide probes, e.g. in the case of rDNA probes, where target stretches containing variable sequences are rather short (about 10-30 nucleotides). The application of probe technologies in molecular environmental studies by in situ hybridization, screening of gene libraries and flow cytometry is in its infancy but a logical step towards reconfirmation of the identity of a species in its natural habitat.
Keyword DNA
DNA probes
gene libraries
molecular microbial ecology
polymerase chain reaction primers
unculturable organisms
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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