Derivatives of the purple phosphatase from red kidney bean: Replacement of zinc with other divalent metal ions

Beck J.L., McArthur M.J., De Jersey J. and Zerner B. (1988) Derivatives of the purple phosphatase from red kidney bean: Replacement of zinc with other divalent metal ions. Inorganica Chimica Acta, 153 1: 39-44. doi:10.1016/S0020-1693(00)83354-X


Author Beck J.L.
McArthur M.J.
De Jersey J.
Zerner B.
Title Derivatives of the purple phosphatase from red kidney bean: Replacement of zinc with other divalent metal ions
Journal name Inorganica Chimica Acta   Check publisher's open access policy
ISSN 0020-1693
Publication date 1988-01-01
Sub-type Article (original research)
DOI 10.1016/S0020-1693(00)83354-X
Open Access Status Not yet assessed
Volume 153
Issue 1
Start page 39
End page 44
Total pages 6
Subject 1303 Specialist Studies in Education
1604 Human Geography
1606 Political Science
2505 Materials Chemistry
Abstract Apoenzyme, containing ≤0.1 zinc atoms and ≤0.2 Fe atoms per subunit and with ≤3% of the phosphatase activity, has been prepared from native red kidney bean purple phosphatase. Treatment of this apoenzyme with Fe3+ or Zn2+ separately gave very little recovery of activity, whereas treatment with both Fe3+ and Zn2+ resulted in complete restoration of activity, indicating that both metal ions are essential. ZnFe enzyme with close to one iron and one zinc atom per subunit has been reconstituted by this procedure. Essentially full reactivation was also achieved by addition of Fe3+ together with Fe2+ or Co2+ to the apoenzyme; Fe3+ and Cd2+ gave 27% restoration of activity, whereas Fe3+ with Mn2+, Cu2+, Ni2+ or Hg2+ gave little or no increase in activity. Kinetic parameters for the hydrolysis of p-nitrophenyl phosphate and ATP by the FeFe derivative are reported.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 50 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 13 Sep 2016, 14:31:32 EST by System User