Subpopulations of splenic T and B lymphocytes producing and regulating leukocyte adherence inhibition factor

Dunn I.S. and Halliday W.J. (1980) Subpopulations of splenic T and B lymphocytes producing and regulating leukocyte adherence inhibition factor. Cellular Immunology, 56 2: 465-477. doi:10.1016/0008-8749(80)90121-5


Author Dunn I.S.
Halliday W.J.
Title Subpopulations of splenic T and B lymphocytes producing and regulating leukocyte adherence inhibition factor
Journal name Cellular Immunology   Check publisher's open access policy
ISSN 1090-2163
Publication date 1980-01-01
Sub-type Article (original research)
DOI 10.1016/0008-8749(80)90121-5
Volume 56
Issue 2
Start page 465
End page 477
Total pages 13
Subject 1307 Cell Biology
2403 Immunology
Abstract Picryl chloride induces contact hypersensitivity in mice, accompanied by spleen cell sensitization that is demonstrable in vitro by specific antigen-induced formation of leukocyte adherence inhibition factor (LAIF). This cellular activity was detected only up to 7 days after sensitization; thereafter the spleen cells appeared to be unreactive with the antigen. The cells were still normally reactive with the mitogen concanavalin A. Antigen reactivity of such "late" cells was restored by passage through a glass-bead column (provided resulting nonadherent cells were reconstituted with normal macrophages), and the restored reactivity was again suppressed by the eluted glass-bead-adherent cells. Suppression was antigen specific. Separation of T and B lymphocytes by affinity chromatography, after glass-bead treatment of sensitized spleen cells, showed that two subpopulations of B cells-those responsible for producing LAIF as well as those suppressing LAIF production by T cells-were glassbead adherent. This was extended by showing directly with anti-Thy-1.2 serum that B cells producing LAIF and suppressor T cells were glass adherent. Thus two suppressive cell populations, and the B cell producing LAIF, were glass adherent while the T-cell LAIF producer was not. Tests for adoptive transfer of cutaneous hypersensitivity in vivo demonstrated the relevance of many of the above observations to conditions in the whole animal. "Late" spleen cells from sensitized mice could not transfer hypersensitivity but this property was restored by glass-bead passage. The eluted adherent cells suppressed transfer. Both adoptive transfer and its suppression were antigen specific.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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