Hydroxyl radical generation following ischaemia-reperfusion in cell-free perfused rat kidney

Kadkhodaee M., Endre Z.H., Towner R.A. and Cross M. (1995) Hydroxyl radical generation following ischaemia-reperfusion in cell-free perfused rat kidney. BBA - General Subjects, 1243 2: 169-174. doi:10.1016/0304-4165(94)00141-J


Author Kadkhodaee M.
Endre Z.H.
Towner R.A.
Cross M.
Title Hydroxyl radical generation following ischaemia-reperfusion in cell-free perfused rat kidney
Journal name BBA - General Subjects   Check publisher's open access policy
ISSN 0304-4165
Publication date 1995-02-23
Sub-type Article (original research)
DOI 10.1016/0304-4165(94)00141-J
Open Access Status Not yet assessed
Volume 1243
Issue 2
Start page 169
End page 174
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract The difficulty in direct detection of oxygen-derived free radicals (OFR) in the intact kidney has left uncertain the role of OFR in renal hypoperfusion injury. Salicylate hydroxylation was used as a sensitive method of estimating the extent of production of highly reactive hydroxyl radicals in renal ischaemia-reperfusion injury in the intact rat kidney perfused with recirculating cell-free medium. The reaction products were detected and quantified by HPLC with electrochemical detection. Hydroxyl radicals were detected as 2,5-dihydroxybenzoic acid (2,5-DHBA). Ischaemia for 15 min followed by reperfusion for 15 min caused more than a twofold increase in 2,5-DHBA concentration (to 2279 ± 225 pg/g tissue weight) compared to controls (933 ± 103, P < 0.001). Addition of 15 mM dimethylthiourea (DMTU) before induction of ischaemia prevented this increase. Induction of hypoxia for 15 min with continued perfusion (as a model of low-flow ischaemia) had no significant effect on hydroxyl radical formation. We conclude that significant quantities of hydroxyl radicals form in the absence of circulating leucocytes during reperfusion following ischaemia, but not during hypoxia in the perfused rat kidney.
Keyword (Isolated perfused rat kidney)
HPLC
Hydroxyl radical
Ischaemia-reperfusion
Salicylate hydroxylation
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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