Pooling and comparing estimates from several experiments of a Michaelis constant for an enzyme

Duggleby R.G. (1990) Pooling and comparing estimates from several experiments of a Michaelis constant for an enzyme. Analytical Biochemistry, 189 1: 84-87. doi:10.1016/0003-2697(90)90048-E


Author Duggleby R.G.
Title Pooling and comparing estimates from several experiments of a Michaelis constant for an enzyme
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1990-08-15
Sub-type Article (original research)
DOI 10.1016/0003-2697(90)90048-E
Volume 189
Issue 1
Start page 84
End page 87
Total pages 4
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Often the Michaelis constant of an enzyme will be determined several times. This may be done for various reasons such as ensuring reproducibility, comparing different enzyme preparations, or examining the effects of variations in experimental conditions. In these circumstances, two questions arise. First, how can the various estimates of the Michaelis constant be compared to determine whether they are the same within the limits of experimental variation? Secondly, if they are all the same, how can the values be combined to give an overall estimate? These questions are addressed here and a solution proposed in which the sets of data are pooled and analyzed with a separate maximum velocity for each set but a common Michaelis constant. The pooled data are partitioned in suitable ways and reanalyzed to examine, by means of a variance ratio test, whether a single Michaelis constant gives a satisfactory fit to the data.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 14 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 13 Sep 2016, 13:49:40 EST by System User