Organogenesis and a general procedure for plant regeneration from callus culture of a commercial duboisia hybrid (D. leichhardtii x D. myoporoides)

Lin G.D. and Griffin W.J. (1992) Organogenesis and a general procedure for plant regeneration from callus culture of a commercial duboisia hybrid (D. leichhardtii x D. myoporoides). Plant Cell Reports, 11 4: 207-210. doi:10.1007/BF00232535


Author Lin G.D.
Griffin W.J.
Title Organogenesis and a general procedure for plant regeneration from callus culture of a commercial duboisia hybrid (D. leichhardtii x D. myoporoides)
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1007/BF00232535
Volume 11
Issue 4
Start page 207
End page 210
Total pages 4
Publisher Springer-Verlag
Subject 1110 Nursing
Abstract The Duboisia hybrid (D. leichhardtii x D. myoporoides) was regenerated from callus derived from shoot tips and young seeds on Murashige and Skoog (MS) medium containing 54μM 1-naphthalenacetic acid (NAA) and 1μM 6-benzylaminopurine (BA). Callus derived from young seeds was superior to shoot tip callus. Buds were induced from callus on MS medium supplemented with 22μM BA. The buds formed shoots when transferred to MS medium with 5 μM BA and 0.5μM NAA. Shoots were induced to form roots when placed on MS medium containing 25 μM indolebutyric acid (IBA). Plantlets so formed were transplanted and subsequently planted out in the open. Approximately 6 months were required for the full regenerative process. Trees so formed have been grown for 3 years, reached a height of 3 metres and flowered and fruited normally.
Keyword callus culture
Duboisia hybrid
organogenesis
regeneration
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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