Effects of epidermal growth factor on preimplantation mouse embryos

Wood S.A. and Kaye P.L. (1989) Effects of epidermal growth factor on preimplantation mouse embryos. Journal of Reproduction and Fertility, 85 2: 575-582. doi:10.1530/jrf.0.0850575

Author Wood S.A.
Kaye P.L.
Title Effects of epidermal growth factor on preimplantation mouse embryos
Journal name Journal of Reproduction and Fertility   Check publisher's open access policy
ISSN 0022-4251
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1530/jrf.0.0850575
Open Access Status Not yet assessed
Volume 85
Issue 2
Start page 575
End page 582
Total pages 8
Language eng
Subject 1309 Developmental Biology
1312 Molecular Biology
1314 Physiology
2710 Embryology
2729 Obstetrics and Gynaecology
Abstract When epidermal growth factor (EGF) was added to the medium for culture of preimplantation embryos, morphological development as determined by microscopic observation was unaffected, but 333 nM-EGF stimulated total uptake of [H]leucine by late morulae/blastocysts which had been cultured for 24 h from morulae. Incorporation of [H]leucine into protein by these embryos was increased by 0.33, 3.3 and 33 nM-EGF, following a quadratic relationship producing less stimulation at 333 nM, which may indicate down regulation of receptors. The estimated EC was ~0.25 nM. Manipulation of the culture period indicated that the embryos responded to EGF at the morula/blastocyst transition period and immunosurgery was used to show that the increased protein synthesis was restricted to the trophectoderm cells. No mitogenic effect was observed. The effective concentration of EGF is close to that of serum and to values which stimulate other tissues. It is suggested that EGF receptors appear at compaction and that EGF may have a role in differentiation of the trophectoderm cells.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Citation counts: Scopus Citation Count Cited 110 times in Scopus Article | Citations
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Created: Tue, 13 Sep 2016, 11:31:49 EST by System User