A Note on the Diagnosis of Ratoon Stunting Disease of Sugarcane by Negative‐Stain Electron Microscopy of the Associated Bacterium

TEAKLE D.S., KONTZE D. and APPLETON J.M. (1979) A Note on the Diagnosis of Ratoon Stunting Disease of Sugarcane by Negative‐Stain Electron Microscopy of the Associated Bacterium. Journal of Applied Bacteriology, 46 2: 279-284. doi:10.1111/j.1365-2672.1979.tb00821.x


Author TEAKLE D.S.
KONTZE D.
APPLETON J.M.
Title A Note on the Diagnosis of Ratoon Stunting Disease of Sugarcane by Negative‐Stain Electron Microscopy of the Associated Bacterium
Journal name Journal of Applied Bacteriology   Check publisher's open access policy
ISSN 1365-2672
Publication date 1979-01-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2672.1979.tb00821.x
Open Access Status
Volume 46
Issue 2
Start page 279
End page 284
Total pages 6
Subject 2402 Applied Microbiology and Biotechnology
2404 Microbiology
2700 Medicine
Abstract After negatively staining with 1% (w/v) sodium phosphotungstate (pH 6·5) or 1% (w/v) ammonium molybdate (pH 6·5), the cell wall, cytoplasmic membrane and mesosomes of the RSD‐associated bacterium obtained from the fibrovascular fluid of infected sugarcane were usually clearly displayed. The cells measured 0·19–0·39 μm (av. 0·27 μm) in width and 0·6–3·4 μm in length. Few mesosomes were visible and the cells were approx. 40% wider (0·27–0·52 μm, av. 0·38 μm) when stained with 1% (w/v) uranyl acetate (pH 3·0–4·2). Freezing and thawing the suspension before negative staining with sodium phosphotungstate did not greatly affect the size of the cells or resolution of the mesosomes. Glycine (0·25 M) as the suspending medium, fixation in 2% (w/w) glutaraldehyde, or placing wet instead of dry specimen grids in the electron microscope resulted in wider cells usually lacking mesosomes. Copyright
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 13 Sep 2016, 11:22:19 EST by System User