Brain angiotensinogen: In vitro synthesis and chromatographic characterization

Sernia C. and Mowchanuk M.D. (1983) Brain angiotensinogen: In vitro synthesis and chromatographic characterization. Brain Research, 259 2: 275-283. doi:10.1016/0006-8993(83)91258-1


Author Sernia C.
Mowchanuk M.D.
Title Brain angiotensinogen: In vitro synthesis and chromatographic characterization
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
Publication date 1983-01-24
Sub-type Article (original research)
DOI 10.1016/0006-8993(83)91258-1
Open Access Status
Volume 259
Issue 2
Start page 275
End page 283
Total pages 9
Subject 1309 Developmental Biology
1312 Molecular Biology
2728 Clinical Neurology
2800 Neuroscience
Abstract The capacity of rat brain to synthesize angiotensinogen (renin substrate) was examined in vitro using brain slices (300 μm thick) incubated in minimum essential medium (Eagles) at pH 7.4 and 37°C for 2 h. The concentration of angiotensinogen (measured as equivalents of angiotensin I; AI) increased from 5.2 ± 1.1 ng AI/g of incubated tissue to 40 ± 4 ng AI/g at 2 h. In the presence of cycloheximide (10 μg/ml) or vinblastine sulfate (10 μg/ml) the values at the 2 h interval were 24.5 ± 3.5 ng AI/g and 10.5 ± 3.5 ng AI/g respectively. The angiotensinogen released by brain slices was pooled and some of its physicochemical properties examined and compared to those of angiotensin from plasma and saline-perfused brains. Chromatography on Sephacryl S-200 showed brain and plasma angiotensinogen to be similar in size (mol. wt. ∼ 59,000) but large differences in binding activity to Concanavalin A were found. Only 31% of plasma angiotensinogen bound to the affinity medium compared to 63% for the pooled incubation medium. It was concluded that de novo synthesis of angiotensinogen occurs in the rat brain protein may differ in its glycoside composition from hepatic angiotensinogen.
Keyword angiotensinogen
biosynthesis
concanavalin A
cycloheximide
rat brain
renin-substrate
vinblastine
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 13 Sep 2016, 11:17:05 EST by System User