Enzyme histochemistry of the sheep uterus during the oestrous cycle

Zamiri M.J. and Blackshaw A.W. (1979) Enzyme histochemistry of the sheep uterus during the oestrous cycle. Australian Journal of Biological Sciences, 32 5: 409-414. doi:10.1071/BI9790409


Author Zamiri M.J.
Blackshaw A.W.
Title Enzyme histochemistry of the sheep uterus during the oestrous cycle
Journal name Australian Journal of Biological Sciences   Check publisher's open access policy
ISSN 0004-9417
Publication date 1979-01-01
Sub-type Article (original research)
DOI 10.1071/BI9790409
Volume 32
Issue 5
Start page 409
End page 414
Total pages 6
Subject 1103 Clinical Sciences
1309 Developmental Biology
1311 Genetics
1305 Biotechnology
1312 Molecular Biology
1310 Endocrinology
2743 Reproductive Medicine
2700 Medicine
Abstract Enzyme histochemical techniques were applied to frozen sheep uteri from different stages of the oestrous cycle. The localization and activities of succinate, lactate, glucose-6-phosphate, and isocitrate (NADP+) dehyrogenases and acid and alkaline phosphatases were studied in the luminal and glandular epithelia, caruncle and myometrium. Enzyme activity in the sections was scored on a scale of 0-5. In general the enzyme activity in the uterine caruncles and epithelia was higher than in the myometrium. The myometrium did not show any alkaline phosphatase activity and isocitrate dehydrogenase (NADP+) activity was negligible. The low activities of acid phosphatase and lactate dehydrogenase and the moderate levels of glucose-6-phosphate and succinate dehydrogenases in the myometrium were constant. The caruncular tissue showed high levels of phosphatases and glucose-6-phosphate dehydrogenase, moderate levels of lactate and succinate dehydrogenases, and low levels of isocitrate dehydrogenase (NADP+) throughout the oestrous cycle. Much lower phosphatase and isocitrate dehydrogenase (NADP+) levels were found in the epithelium of deep glands compared with superficial glands. The high activity of acid and alkaline phosphatases in the luminal epithelium and the superficial glands was constant from mid-cycle to ovulation, but a significant decrease was observed immediately after ovulation. The level of dehydrogenases in epithelia was generally high and did not change during the oestrous cycle.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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