The effect of mycoplasmas and acholeplasmas of equine origin on organ cultures of chicken-embryo trachea

Moorthy A.R.S. and Spradbrow P.B. (1985) The effect of mycoplasmas and acholeplasmas of equine origin on organ cultures of chicken-embryo trachea. Journal of Comparative Pathology, 95 2: 209-216. doi:10.1016/0021-9975(85)90007-6


Author Moorthy A.R.S.
Spradbrow P.B.
Title The effect of mycoplasmas and acholeplasmas of equine origin on organ cultures of chicken-embryo trachea
Journal name Journal of Comparative Pathology   Check publisher's open access policy
ISSN 0021-9975
Publication date 1985-01-01
Sub-type Article (original research)
DOI 10.1016/0021-9975(85)90007-6
Volume 95
Issue 2
Start page 209
End page 216
Total pages 8
Subject 1103 Clinical Sciences
Abstract Chicken-embryo tracheal organ cultures were inoculated with equine strains of Mycoplasma arginini, M. equigenitalium, 2 strains of M. subdolum, Acholeplasma laidwii and 3 strains of A. oculi. All strains established and multiplied in the explant cultures, but only M. subdolum and A. oculi produced a cytopathic effect on ciliated epithelial cells, causing sloughing of cells and cilia after 6 days. There was a correlation between ciliostasis and increase in titre of both M. subdolum and A. oculi and this relationship was not observed with M. equigenitalium and A. laidlawii. All the strains of acholeplasma multiplied to some extent in organ culture media, but reached higher titres in the presence of explants. Cells infected with the M. subdolum strain showed sloughing of cilia, vacuolization, and increase in size of mitochondria, followed by disorganization of epithelium and marked destruction of subcellular organelles. Mycoplasmas were closely attached to the epithelial surface of the tracheal explant 8 days after infection.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 13 Sep 2016, 11:02:37 EST by System User