Organophosphate inhibitors The stereospecificity of hydrolysis of methyl n-butyl p-nitrophenyl phosphate by serum phosphotriesterases (EC 3.1.1.2) and by acetylcholinesterases (EC 3.1.1.7)

Ddudman N.P.B., De Jersey J. and Zerner B. (1977) Organophosphate inhibitors The stereospecificity of hydrolysis of methyl n-butyl p-nitrophenyl phosphate by serum phosphotriesterases (EC 3.1.1.2) and by acetylcholinesterases (EC 3.1.1.7). BBA - Enzymology, 481 1: 127-139. doi:10.1016/0005-2744(77)90144-9


Author Ddudman N.P.B.
De Jersey J.
Zerner B.
Title Organophosphate inhibitors The stereospecificity of hydrolysis of methyl n-butyl p-nitrophenyl phosphate by serum phosphotriesterases (EC 3.1.1.2) and by acetylcholinesterases (EC 3.1.1.7)
Journal name BBA - Enzymology
ISSN 0005-2744
Publication date 1977-03-15
Sub-type Article (original research)
DOI 10.1016/0005-2744(77)90144-9
Open Access Status
Volume 481
Issue 1
Start page 127
End page 139
Total pages 13
Subject 2700 Medicine
Abstract A chiral phosphotriester, methyl n-butylp-nitrophenyl phosphate was ased to determine the stereospecificity of hydrolysis catalysed by serum phosphotriesterases (aryl-ester hydrolases, EC 3.1.1.2) from horse, ox and rabbit. Each enzyme hydrolysed the (-)-enantiomer more quickly. The same phosphate was used to inhibit acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) from ox, rabbit and electric eel, and in each case, the (+)-enantiomer caused more rapid inhibition. Serum phosphotriesterase did not catalyse the dephosphorylation of dialkylphosphoryl-acetylcholinesterase or dialkylphosphorylcarboxylesterase. Levels of serum phosphotriesterase in rabbits which received sub-lethal injections of the phosphotriester remained unchanged after one or several injections. In the same rabbits, the levels of blood acetylcholinesterase fell sharply following injections, but normal values were regained in 2-8 days. Serum phosphotriesterases seem incapable either of preventing acute phosphotriester poisoning or of regenerating active enzyme from phosphorylated acetylcholinesterase. However, phosphotriesterases would act in cases of chronic exposure by catalysing the hydrolysis of such organophosphate poisons as remain in the blood.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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