Comparative pathogenesis of eosinophilic meningitis caused by Angiostrongylus mackerrasae and Angiostrongylus cantonensis in murine and guinea pig models of human infection

Aghazadeh, Mahdis, Harvie, Marina C., Owen, Helen C., Verissimo, Carolina, Aland, Kieran V., Reid, Simon A., Traub, Rebecca J., McManus, Donald P., McCarthy, James S. and Jones, Malcolm K. (2016) Comparative pathogenesis of eosinophilic meningitis caused by Angiostrongylus mackerrasae and Angiostrongylus cantonensis in murine and guinea pig models of human infection. Parasitology, 143 10: 1243-1251. doi:10.1017/S003118201600069X


Author Aghazadeh, Mahdis
Harvie, Marina C.
Owen, Helen C.
Verissimo, Carolina
Aland, Kieran V.
Reid, Simon A.
Traub, Rebecca J.
McManus, Donald P.
McCarthy, James S.
Jones, Malcolm K.
Title Comparative pathogenesis of eosinophilic meningitis caused by Angiostrongylus mackerrasae and Angiostrongylus cantonensis in murine and guinea pig models of human infection
Formatted title
Comparative pathogenesis of eosinophilic meningitis caused by Angiostrongylus mackerrasae and Angiostrongylus cantonensis in murine and guinea pig models of human infection
Journal name Parasitology   Check publisher's open access policy
ISSN 1469-8161
0031-1820
Publication date 2016-09-01
Year available 2016
Sub-type Article (original research)
DOI 10.1017/S003118201600069X
Open Access Status Not yet assessed
Volume 143
Issue 10
Start page 1243
End page 1251
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Cambridge University Press
Language eng
Abstract This study investigated comparatively the pathogenicity of experimental infection of mice and guinea pigs, with Angiostrongylus mackerrasae and the closely related species A. cantonensis. Time course analyses showed that A. mackerrasae causes eosinophilic meningitis in these hosts, which suggests that the species has the potential to cause meningitis in humans and domestic animals. Both A. mackerrasae and the genetically similar A. cantonensis caused eosinophilic meningitis in mice at two time points of 14 and 21 days post infection (dpi). The brain lesions in mice infected with A. mackerrasae were more granulomatous in nature and the parasites were more likely to appear degenerate compared with lesions caused by A. cantonensis. This may indicate that the mouse immune system eliminates A. mackerrasae infection more effectively. The immunologic responses of mice infected with the two Angiostrongylus species was compared by assessing ex vivo stimulated spleen derived T cells and cytokines including interferon-gamma, interleukin 4 and interleukin 17 on 14 and 21 dpi. The results were similar for mice infected with A. cantonensis and A. mackerrasae. Serum from the infected animals with either A. cantonensis or A. mackerrasae recognized total soluble antigen of A. cantonensis female worms on Western blot.
Formatted abstract
This study investigated comparatively the pathogenicity of experimental infection of mice and Guinea pigs, with Angiostrongylus mackerrasae and the closely related species A. cantonensis. Time course analyses showed that A. mackerrasae causes eosinophilic meningitis in these hosts, which suggests that the species has the potential to cause meningitis in humans and domestic animals. Both A. mackerrasae and the genetically similar A. cantonensis caused eosinophilic meningitis in mice at two time points of 14 and 21 days post infection (dpi). The brain lesions in mice infected with A. mackerrasae were more granulomatous in nature and the parasites were more likely to appear degenerate compared with lesions caused by A. cantonensis. This may indicate that the mouse immune system eliminates A. mackerrasae infection more effectively. The immunologic responses of mice infected with the two Angiostrongylus species was compared by assessing ex vivo stimulated spleen derived T cells and cytokines including interferon-gamma, interleukin 4 and interleukin 17 on 14 and 21 dpi. The results were similar for mice infected with A. cantonensis and A. mackerrasae. Serum from the infected animals with either A. cantonensis or A. mackerrasae recognized total soluble antigen of A. cantonensis female worms on Western blot.
Keyword Angiostrongyliasis
Angiostrongylus cantonensis
Angiostrongylus mackerrasae
Eosinophilic meningitis
Rat lungworm
Rattus fuscipes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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