Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells

Jahidin, Aisyah H., Stewart, Teneale A., Thompson, Erik W., Roberts-Thomson, Sarah J. and Monteith, Gregory R. (2016) Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells. Biochemical and Biophysical Research Communications, 477 4: 731-736. doi:10.1016/j.bbrc.2016.06.127

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Author Jahidin, Aisyah H.
Stewart, Teneale A.
Thompson, Erik W.
Roberts-Thomson, Sarah J.
Monteith, Gregory R.
Title Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
0006-291X
Publication date 2016-09-02
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2016.06.127
Open Access Status File (Author Post-print)
Volume 477
Issue 4
Start page 731
End page 736
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated. TPC1 and TPC2 mRNA was present in all non-tumorigenic and tumorigenic breast cell lines assessed. Silencing of TPC2 but not TPC1 attenuated epidermal growth factor-induced vimentin expression in MDA-MB-468 breast cancer cells. This effect was not due to a general inhibition of epithelial to mesenchymal transition (EMT) as TPC2 silencing had no effect on epidermal growth factor (EGF)-induced changes on E-cadherin expression. TPC1 and TPC2 were also shown to differentially regulate cyclopiazonic acid (CPA)-mediated changes in cytosolic free Ca2+. These findings indicate potential differential regulation of signaling processes by TPC1 and TPC2 in breast cancer cells.
Keyword Breast cancer
Calcium
Calcium signaling
Epidermal growth factor
Two-pore channel
Vimentin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
HERDC Pre-Audit
School of Pharmacy Publications
 
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