Field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens

Lau, Han Yih, Wang, Yuling, Wee, Eugene J. H., Botella, Jose R. and Trau, Matt (2016) Field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens. Analytical Chemistry, 88 16: 8074-8081. doi:10.1021/acs.analchem.6b01551


Author Lau, Han Yih
Wang, Yuling
Wee, Eugene J. H.
Botella, Jose R.
Trau, Matt
Title Field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 0003-2700
1520-6882
Publication date 2016-08-16
Year available 2016
Sub-type Article (original research)
DOI 10.1021/acs.analchem.6b01551
Open Access Status Not yet assessed
Volume 88
Issue 16
Start page 8074
End page 8081
Total pages 8
Place of publication Washington, DC United States
Publisher American Chemical Society
Language eng
Subject 1602 Analytical Chemistry
Abstract Effective disease management strategies to prevent catastrophic crop losses require rapid, sensitive, and multiplexed detection methods for timely decision making. To address this need, a rapid, highly specific and sensitive point of -care method for multiplex detection of plant pathogens was developed by taking advantage of surface-enhanced Raman scattering (SERS) labeled nanotags and recombinase polymerase amplification (RPA), which is a rapid isothermal amplification method with high specificity. In this study, three agriculturally important plant pathogens (Botrytis cinerea, Pseudomonas syringae, and Fusarium oxysporum) were used to demonstrate potential translation into the field. The RPA-SERS method was faster, more sensitive than polymerase chain reaction, and could detect as little as 2 copies of B. cinerea DNA. Furthermore, multiplex detection of the three pathogens was demonstrated for complex systems such as the Arabidopsis thaliana plant and commercial tomato crops. To demonstrate the potential for on-site field applications, a rapid single-tube RPA/SERS assay was further developed and successfully performed for a specific target outside of a laboratory setting.
Formatted abstract
Effective disease management strategies to prevent catastrophic crop losses require rapid, sensitive, and multiplexed detection methods for timely decision making. To address this need, a rapid, highly specific and sensitive point-of-care method for multiplex detection of plant pathogens was developed by taking advantage of surface-enhanced Raman scattering (SERS) labeled nanotags and recombinase polymerase amplification (RPA), which is a rapid isothermal amplification method with high specificity. In this study, three agriculturally important plant pathogens (Botrytis cinerea, Pseudomonas syringae, and Fusarium oxysporum) were used to demonstrate potential translation into the field. The RPA-SERS method was faster, more sensitive than polymerase chain reaction, and could detect as little as 2 copies of B. cinerea DNA. Furthermore, multiplex detection of the three pathogens was demonstrated for complex systems such as the Arabidopsis thaliana plant and commercial tomato crops. To demonstrate the potential for on-site field applications, a rapid single-tube RPA/SERS assay was further developed and successfully performed for a specific target outside of a laboratory setting.
Keyword Chemistry, Analytical
Chemistry
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 2014002940
CG-08-07
Institutional Status UQ

 
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