Plasmodium berghei ANKA infection in ICR mice as a model of cerebral malaria

Basir, R., Rahiman, S.S.F., Hasballah, K., Chong, W.C., Talib, H., Yam, M.F., Jabbarzare, M., Tie, T.H., Othman, F., Moklas, M.A.M., Abdullah, W.O. and Ahmad, Z. (2012) Plasmodium berghei ANKA infection in ICR mice as a model of cerebral malaria. Iranian Journal of Parasitology, 7 4: 62-74.

Author Basir, R.
Rahiman, S.S.F.
Hasballah, K.
Chong, W.C.
Talib, H.
Yam, M.F.
Jabbarzare, M.
Tie, T.H.
Othman, F.
Moklas, M.A.M.
Abdullah, W.O.
Ahmad, Z.
Title Plasmodium berghei ANKA infection in ICR mice as a model of cerebral malaria
Journal name Iranian Journal of Parasitology   Check publisher's open access policy
ISSN 1735-7020
Publication date 2012-10-01
Year available 2012
Sub-type Article (original research)
Open Access Status Link (no DOI)
Volume 7
Issue 4
Start page 62
End page 74
Total pages 13
Place of publication Tehran, Islamic Republic of Iran
Publisher Tehran University of Medical Sciences Publications
Formatted abstract
Background: Animal models with various combination of host-parasite have long been employed to study malaria pathogenesis. Here, we describe the combination of Plasmodium berghei ANKA infection in inbred ICR mice as a model of cerebral malaria (CM).

Infection in mice was initiated by intraperitoneal injection of 2 x 10(7) (0.2ml) parasitized red blood cells (PRBCs).

Results: This model can produce a severe degree of infection presented by the high degree of parasitaemia followed by death 6-7 days post infection. Severe anemia, splenomegaly, hepatomegaly and discolourations of major organs were observed. Histopathological findings revealed several important features mimicking human CM including, microvascular sequestration of PRBCs in major organs, particularly in the brain, hypertrophy and hyperplasia of the kupffer cells in the liver, pulmonary edema and hyaline membrane formation in the lungs and haemorrhages in the kidney's medulla and cortex. Proinflammatory cytokines TNF alpha, IFN gamma, IL-1, IL-6 and IL-18, and anti-inflammatory cytokine IL-10 were all found to be elevated in the plasma of infected mice.

Conclusion: This model can reproduce many of the important features of CM and therefore can be used as a tool to advance our understanding of the disease pathogenesis.
Keyword Animal model
ICR mice
Plasmodium berghei
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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