Hybridoma growth limitations: The roles of energy metabolism and ammonia production

Newland M., Greenfield P.F. and Reid S. (1990) Hybridoma growth limitations: The roles of energy metabolism and ammonia production. Cytotechnology, 3 3: 215-229. doi:10.1007/BF00365485


Author Newland M.
Greenfield P.F.
Reid S.
Title Hybridoma growth limitations: The roles of energy metabolism and ammonia production
Journal name Cytotechnology   Check publisher's open access policy
ISSN 0920-9069
Publication date 1990-01-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1007/BF00365485
Open Access Status
Volume 3
Issue 3
Start page 215
End page 229
Total pages 15
Publisher Kluwer Academic Publishers
Subject 1308 Clinical Biochemistry
1307 Cell Biology
1305 Biotechnology
2700 Medicine
Abstract Energy metabolism and the production of ammonia in hybridoma cell culture and its inhibitory effects on cell growth are reviewed. The interactive roles of glucose and glutamine metabolism affect the rate of production of ammonia, and these interactions are described. It is shown that growth inhibition usually occurs between 2-4 mM ammonia although some cell lines have been shown to adapt to much higher concentrations, particularly in continuous culture. In batch cultures cell growth appears to be particularly susceptible to increased ammonia concentrations during the early stages of growth; ammonia increased the rate of cell death in the late stage of batch growth. The specific productivity of monoclonal antibodies is much less sensitive to the released ammonia than is growth; lower volumetric productivities relate to the lower viable cell concentrations which are achieved at the high ammonia levels. Techniques to prevent ammonia accumulation or remove ammonia selectively have been relatively unsuccessful to date.
Keyword ammonia
ammonia removal
energetics
glutaminolysis
hybridoma
inhibition
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Scopus Import - Archived
 
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Created: Tue, 06 Sep 2016, 12:05:47 EST by System User