Ultrastructural immunoperoxidase investigations of human glomerular cells in tissue culture

Jackson A.E., Atkins R.C. and Glasgow E.F. (1989) Ultrastructural immunoperoxidase investigations of human glomerular cells in tissue culture. Nephron, 52 1: 62-71.

Author Jackson A.E.
Atkins R.C.
Glasgow E.F.
Title Ultrastructural immunoperoxidase investigations of human glomerular cells in tissue culture
Journal name Nephron   Check publisher's open access policy
ISSN 0028-2766
Publication date 1989-01-01
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 52
Issue 1
Start page 62
End page 71
Total pages 10
Language eng
Subject 2727 Nephrology
Abstract In order to elucidate the cells produced from human glomerular cultures, a pre-embedding ultrastructural immunoperoxidase technique was developed to investigate the precise localisation of a panel of monoclonal antibodies specific to human glomerular antigens in situ and used to identify the same antigens in vitro. The results obtained were correlated with the morphological features of the cells demonstrated by scanning electron microscopy. The study was able to show the presence of epithelial and mesangial cells in tissue culture, to confirm previous results indicating that glomerular endothelial cells are not present and to give some insight into the production of different basement membrane components by different cell types.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 06 Sep 2016, 11:33:26 EST by System User