A device for aeration and mixing of cell and organelle suspensions during nuclear magnetic resonance studies

Bourne R.M. (1989) A device for aeration and mixing of cell and organelle suspensions during nuclear magnetic resonance studies. Analytical Biochemistry, 182 1: 151-156. doi:10.1016/0003-2697(89)90733-1


Author Bourne R.M.
Title A device for aeration and mixing of cell and organelle suspensions during nuclear magnetic resonance studies
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1016/0003-2697(89)90733-1
Open Access Status Not yet assessed
Volume 182
Issue 1
Start page 151
End page 156
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A device is described which maintains homogeneous aerobic or anaerobic cell and organelle suspensions within an NMR sample tube. Line broadening due to magnetic field inhomogeneity is reduced by elimination of gas bubbles from the area of the probe receiver coils. The linewidth of the extracellular orthophosphate resonance of a yeast suspension in 31P NMR was 0.21 ppm compared with 0.4 - 0.7 ppm in conventionally aerated suspensions. Recirculation of the sample results in complete mixing within 90 s of addition of aliquots of acid or alkali. The maximum rate of oxygen transfer from the gaseous to the liquid phase was approximately 600 μm min-1 when aerated with 95% oxygen 5% carbon dioxide. A 60% wet weight suspension of yeast cells was recirculated for 20 h without settling of cells occurring. A method for estimating oxygen transfer rate is described.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 06 Sep 2016, 10:49:47 EST by System User