Stabilisation of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes

Impoolsup A., Caunt P. and Greenfield P.F. (1989) Stabilisation of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes. Biotechnology Letters, 11 9: 605-608. doi:10.1007/BF01025266


Author Impoolsup A.
Caunt P.
Greenfield P.F.
Title Stabilisation of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes
Journal name Biotechnology Letters   Check publisher's open access policy
ISSN 0141-5492
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1007/BF01025266
Volume 11
Issue 9
Start page 605
End page 608
Total pages 4
Publisher Kluwer Academic Publishers
Subject 2404 Microbiology
2402 Applied Microbiology and Biotechnology
1502 Banking, Finance and Investment
1305 Biotechnology
Abstract The 2μm based yeast plasmid, pLG669-z, was stably maintained in a S. cerevisiae strain during continuous culture with cyclic growth rate changes. In the absence of a selective mechanism, the fraction of plasmid containing cells remained high, and even without optimisation, high gene expression levels could be obtained.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 06 Sep 2016, 10:46:04 EST by System User