Evaluation of hepatocellular and Kupffer cell function of the isolated guinea pig liver perfused by a fluorocarbon medium

Roeser H.P., Cooksley W.G.E. and Mack S. (1978) Evaluation of hepatocellular and Kupffer cell function of the isolated guinea pig liver perfused by a fluorocarbon medium. Gastroenterology, 74 2 PART 1: 209-214.

Author Roeser H.P.
Cooksley W.G.E.
Mack S.
Title Evaluation of hepatocellular and Kupffer cell function of the isolated guinea pig liver perfused by a fluorocarbon medium
Journal name Gastroenterology   Check publisher's open access policy
ISSN 0016-5085
Publication date 1978-01-01
Sub-type Article (original research)
Volume 74
Issue 2 PART 1
Start page 209
End page 214
Total pages 6
Subject 2715 Gastroenterology
Abstract A synthetic medium for isolated guinea pig liver perfusion, using a fluorocarbon (FC) emulsion as the oxygen-carrying agent, was evaluated for its capacity to maintain liver viability and function. A 10% emulsion of perfluorotributylamine (FC43) was dispersed by a nonionic detergent (Pluronic F68) in a buffered electrolyte solution. The FC43 perfusate was compared with identical media in which FC43 was either omitted or replaced by 20% isologous red cells. Damage to liver cells was assessed by hepatic flow rate, release of aspartate aminotransferase, and liver histology. Bile secretion, albumin synthesis, and galactose elimination capacity were used as indices of parenchymal cell function, and Kupffer cell activity was assessed by uptake of colloidal radioactive gold. All three perfusion media satisfactorily maintained liver cell viability, but the two media supplemented with a specific oxygen-carrying agent were associated with significantly superior parenchymal cell function (P was between 0.05 and 0.01). The performance of the FC and red cell media was not significantly different. The phagocytic activity of Kupffer cells was well sustained by all three media, but the FC medium was superior with large amounts of colloidal gold. Release of iron from hepatic parenchymal cells, with the FC medium, was similar to that reported for rat liver preparations, using a red cell perfusate.
Q-Index Code C1
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 06 Sep 2016, 10:38:34 EST by System User