Isolation and expansion of mesenchymal stem/stromal cells derived from human placenta tissue

Pelekanos, Rebecca A., Sardesai, Varda S., Futrega, Kathryn, Lott, William B., Kuhn, Michael and Doran, Michael R. (2016) Isolation and expansion of mesenchymal stem/stromal cells derived from human placenta tissue. Journal of Visualized Experiments, 2016 112: 1-13. doi:10.3791/54204


Author Pelekanos, Rebecca A.
Sardesai, Varda S.
Futrega, Kathryn
Lott, William B.
Kuhn, Michael
Doran, Michael R.
Title Isolation and expansion of mesenchymal stem/stromal cells derived from human placenta tissue
Journal name Journal of Visualized Experiments   Check publisher's open access policy
ISSN 1940-087X
Publication date 2016-06-06
Year available 2016
Sub-type Article (original research)
DOI 10.3791/54204
Open Access Status DOI
Volume 2016
Issue 112
Start page 1
End page 13
Total pages 13
Publisher Journal of Visualized Experiments
Collection year 2017
Language eng
Abstract Mesenchymal stem/stromal cells (MSC) are promising candidates for use in cell-based therapies. In most cases, therapeutic response appears to be cell-dose dependent. Human term placenta is rich in MSC and is a physically large tissue that is generally discarded following birth. Placenta is an ideal starting material for the large-scale manufacture of multiple cell doses of allogeneic MSC. The placenta is a fetomaternal organ from which either fetal or maternal tissue can be isolated. This article describes the placental anatomy and procedure to dissect apart the decidua (maternal), chorionic villi (fetal), and chorionic plate (fetal) tissue. The protocol then outlines how to isolate MSC from each dissected tissue region, and provides representative analysis of expanded MSC derived from the respective tissue types. These methods are intended for pre-clinical MSC isolation, but have also been adapted for clinical manufacture of placental MSC for human therapeutic use.
Keyword Developmental biology
Issue 112
Placenta
Human
Term
Third trimester
Maternal
Mesenchymal stem cell
Stromal cell
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
UQ Centre for Clinical Research Publications
HERDC Pre-Audit
Admin Only - UQCCR
 
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