Methods for assessing the electromechanical integration of human pluripotent stem cell-derived cardiomyocyte grafts

Zhu, Wei-Zhong, Filice, Dominic, Palpant, Nathan J. and Laflamme, Michael A. (2014). Methods for assessing the electromechanical integration of human pluripotent stem cell-derived cardiomyocyte grafts. In Radisci, Milica and Black III, Lauren D. (Ed.), Cardiac tissue engineering: methods and protocols (pp. 229-247) New York, NY United States: Springer New York. doi:10.1007/978-1-4939-1047-2_20


Author Zhu, Wei-Zhong
Filice, Dominic
Palpant, Nathan J.
Laflamme, Michael A.
Title of chapter Methods for assessing the electromechanical integration of human pluripotent stem cell-derived cardiomyocyte grafts
Title of book Cardiac tissue engineering: methods and protocols
Place of Publication New York, NY United States
Publisher Springer New York
Publication Year 2014
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-4939-1047-2_20
Open Access Status Not yet assessed
Series Methods in Molecular Biology
ISBN 9781493910465
9781493910472
ISSN 1064-3745
1940-6029
Editor Radisci, Milica
Black III, Lauren D.
Volume number 1181
Chapter number 20
Start page 229
End page 247
Total pages 19
Total chapters 21
Language eng
Abstract/Summary Cardiomyocytes derived from human pluripotent stem cells show tremendous promise for the replacement of myocardium and contractile function lost to infarction. However, until recently, no methods were available to directly determine whether these stem cell-derived grafts actually couple with host myocardium and fire synchronously following transplantation in either intact or injured hearts. To resolve this uncertainty, our group has developed techniques for the intravital imaging of hearts engrafted with stem cell-derived cardiomyocytes that have been modified to express the genetically encoded protein calcium sensor, GCaMP. When combined with the simultaneously recorded electrocardiogram, this protocol allows one to make quantitative assessments as to the presence and extent of host-graft electrical coupling as well as the timing and pattern of graft activation. As described here, this system has been employed to investigate the electromechanical integration of human embryonic stem cell-derived cardiomyocytes in a guinea pig model of cardiac injury, but analogous approaches should be applicable to other human graft cell types and animal models.
Keyword Human pluripotent stem cells
Zinc finger nuclease
GCaMP3
Cardiac repair
Intravital imaging
Electromechanical coupling
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 30 Aug 2016, 20:50:25 EST by Anthony Yeates on behalf of Institute for Molecular Bioscience