The effect of oxidative injury on hepatocellular influx of leucine in the rat

Le Couteur D.G., Rivory L.P. and Pond S.M. (1996) The effect of oxidative injury on hepatocellular influx of leucine in the rat. International Hepatology Communications, 4 6: 343-350. doi:10.1016/0928-4346(96)00266-6


Author Le Couteur D.G.
Rivory L.P.
Pond S.M.
Title The effect of oxidative injury on hepatocellular influx of leucine in the rat
Journal name International Hepatology Communications
ISSN 0928-4346
Publication date 1996-01-01
Sub-type Article (original research)
DOI 10.1016/0928-4346(96)00266-6
Volume 4
Issue 6
Start page 343
End page 350
Total pages 8
Publisher Elsevier Ireland Ltd
Language eng
Subject 2721 Hepatology
Abstract The hepatocellular influx of leucine was investigated in the perfused rat liver using the multiple indicator-dilution technique. The cellular influx of leucine was saturable and the K(m) and V(max) values were 5.3 ± 1.8 mM and 194 ± 25 nmol/s/g of liver, respectively. These were similar to those reported in cell membrane preparations. Previous studies have indicated that oxidative injury impairs the activity of membrane transporters. Perfusion with 10 mM hydrogen peroxide for 3 min caused a significant reduction in leucine influx from 7.4 ± 1.4 nmol/s/g of liver to 4.6 ± 1.0 nmol/s/g of liver (P = 0.007) with a perfusate leucine concentration of 0.13 μM. This was not associated with any significant change in the extracellular volume (0.31 ± 0.08 ml/g of liver vs. 0.32 ± 0.01 ml/g of liver). This confirms that oxidative injury in the liver is associated with impairment of membrane transport and extends the observation to the transport of leucine.
Keyword Leucine transport
Liver
Multiple indicator-dilution
Oxidative injury
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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