Isolation of manganese-oxidizing Pedomicrobium cultures from water by micromanipulation

Sly L.I. and Arunpairojana V. (1987) Isolation of manganese-oxidizing Pedomicrobium cultures from water by micromanipulation. Journal of Microbiological Methods, 6 3: 177-182. doi:10.1016/0167-7012(87)90013-3


Author Sly L.I.
Arunpairojana V.
Title Isolation of manganese-oxidizing Pedomicrobium cultures from water by micromanipulation
Journal name Journal of Microbiological Methods   Check publisher's open access policy
ISSN 0167-7012
Publication date 1987-01-01
Sub-type Article (original research)
DOI 10.1016/0167-7012(87)90013-3
Open Access Status Not yet assessed
Volume 6
Issue 3
Start page 177
End page 182
Total pages 6
Subject 1305 Biotechnology
2402 Applied Microbiology and Biotechnology
2404 Microbiology
Abstract A method for the isolation of manganese-oxidizing pedomicrobia from water is described. The method employs a combination of growth on a manganese containing indicator medium and micromanipulation. Pedomicrobia are slow-growing and are frequently overgrown by spreading bacteria. Colonies of manganese-oxidizing strains are easily detected at an early stage of development because of the accumulation of black manganese oxide. Micromanipulation allows selection of cells from microcolonies before they are overgrown. The procedure has been successfully used to isolate manganese-oxidizing Pedomicrobium cultures from water distribution systems experiencing persistent manganese-related dirty water problems. Considerable savings in time and materials have been made compared with conventional dilution plating techniques.
Keyword Manganese
Micromanipulation
Pedomicrobium
Water
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 30 Aug 2016, 13:35:07 EST by System User