A procedure for the purification of ferritin from human liver by heating a methanol-treated homogenate

Cham B.E., Roeser H.P., Nikles A. and Ridgway K. (1985) A procedure for the purification of ferritin from human liver by heating a methanol-treated homogenate. Analytical Biochemistry, 151 2: 561-565. doi:10.1016/0003-2697(85)90220-9


Author Cham B.E.
Roeser H.P.
Nikles A.
Ridgway K.
Title A procedure for the purification of ferritin from human liver by heating a methanol-treated homogenate
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1985-01-01
Sub-type Article (original research)
DOI 10.1016/0003-2697(85)90220-9
Open Access Status Not yet assessed
Volume 151
Issue 2
Start page 561
End page 565
Total pages 5
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A simple, rapid technique for purification of ferritin from human liver tissue is described. Methanol, at a final concentration of 40% ( v v) in liver homogenate, precipitates the majority of proteins but does not affect ferritin. Subsequent heating of this homogenate at 75°C for 10 min results in a purified ferritin preparation as judged by immunoelectrophoresis and polyacrylamide gel electrophoresis. The resultant purified ferritin contained the same amount of iron as the original endogenous ferritin. There were no significant differences (paired t tests) in the amount of protein in the purified ferritin preparation when measured by rocket immunoelectrophoresis and by the Lowry procedure, suggesting that the antigenecity of ferritin was unaffected by the methanol and heat treatment. Both endogenous liver ferritin and radiolabeled human liver ferritin added to liver homogenates were recovered after methanol and heat treatment with similar yields (77 ± 7% and 70 ± 2%, respectively) when compared with the standard treatment of heating a homogenate at 75°C. The overall ferritin yield with this rapid procedure was 40%.
Keyword ferritin
human
iron
liver
methanol
purification
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 30 Aug 2016, 13:30:45 EST by System User