Data analysis of plasmid stability in continuous culture of recombinant Saccharomyces cerevisiae

Syamsu K., Impoolsup A., Greenfield P.F. and Mitchell D.A. (1992) Data analysis of plasmid stability in continuous culture of recombinant Saccharomyces cerevisiae. Biotechnology Techniques, 6 5: 393-398. doi:10.1007/BF02447476


Author Syamsu K.
Impoolsup A.
Greenfield P.F.
Mitchell D.A.
Title Data analysis of plasmid stability in continuous culture of recombinant Saccharomyces cerevisiae
Journal name Biotechnology Techniques   Check publisher's open access policy
ISSN 0951-208X
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1007/BF02447476
Volume 6
Issue 5
Start page 393
End page 398
Total pages 6
Publisher Kluwer Academic Publishers
Subject 2402 Applied Microbiology and Biotechnology
1303 Specialist Studies in Education
Abstract A simple method using non-linear regression is developed to analyse experimental data from plasmid stability studies of recombinant Saccharomyces cerevisiae grown in continuous cultures with non-selective and selective media. This method simultaneously provides quantitative information on the probability of plasmid loss due to segregation during cell division and the specific growth rates of plasmid-containing and plasmid-free cells at particular dilution rates. The method is applied to a set of experimental data. The three-parameter model, together with the estimated parameter values, provides a good fit to the experimental data.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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