Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased

Bell L. and Williams L. (1979) Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased. Histochemistry, 60 1: 85-89. doi:10.1007/BF00495731


Author Bell L.
Williams L.
Title Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased
Journal name Histochemistry   Check publisher's open access policy
ISSN 0301-5564
Publication date 1979-01-01
Sub-type Article (original research)
DOI 10.1007/BF00495731
Open Access Status Not yet assessed
Volume 60
Issue 1
Start page 85
End page 89
Total pages 5
Publisher Springer-Verlag
Subject 2702 Anatomy
2700 Medicine
Abstract Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4° C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 μ, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 μ. The simultaneous coupling azo dye method using the substrates sodium α-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Scopus Citation Count Cited 22 times in Scopus Article | Citations
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