Use of a simple γ flow cell to monitor chromatography column effluents containing 75Se

Asher C.J. and Prescott R.J. (1975) Use of a simple γ flow cell to monitor chromatography column effluents containing 75Se. Analytical Biochemistry, 69 2: 618-622. doi:10.1016/0003-2697(75)90166-9


Author Asher C.J.
Prescott R.J.
Title Use of a simple γ flow cell to monitor chromatography column effluents containing 75Se
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1975-01-01
Sub-type Article (original research)
DOI 10.1016/0003-2697(75)90166-9
Volume 69
Issue 2
Start page 618
End page 622
Total pages 5
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Scintillation flow cells provide a convenient means of monitoring column effluents for radioactively labeled compounds. The use of flow cells to monitor effluents containing 3H, 14C, and other β-emitting isotopes is well established (1) and such cells are readily available as accessories for use with liquid scintillation counters. β-Flow cells have occasionally been used to monitor γ emitters [e.g., Martin et al. (2)] but the counting efficiency is extremely low even for weak γ emitters such as 75Se, due to the limited mass of scintillant available to absorb the radiation. Spencer et al. (3) described a simple γ flow cell consisting of a coiled polyethylene tube inserted in to the well crystal of a γ counter, but no information on counting efficiency was given. Although spiral plastic γ cells have been manufactured commercially (e.g., Nuclear Enterprises Ltd., NE7021 1 N.B.: Recent advice from Nuclear Enterprises Ltd., indicates that this γ flow cell is now out of production.) they are not offered as a standard accessory for most common makes of γ counter and do not appear to be readily available from commercial sources. This communication describes a simple, low cost γ flow cell constructed for use in a study on selenium transport in higher plants, which involved the separation of 75Se-labeled constituents in a large number of samples of xylem sap.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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