The coexistence of neuropeptides in feline sensory neurons

Cameron A.A., Leah J.D. and Snow P.J. (1988) The coexistence of neuropeptides in feline sensory neurons. Neuroscience, 27 3: 969-979. doi:10.1016/0306-4522(88)90200-X


Author Cameron A.A.
Leah J.D.
Snow P.J.
Title The coexistence of neuropeptides in feline sensory neurons
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
Publication date 1988-01-01
Sub-type Article (original research)
DOI 10.1016/0306-4522(88)90200-X
Open Access Status Not yet assessed
Volume 27
Issue 3
Start page 969
End page 979
Total pages 11
Subject 2800 Neuroscience
Abstract The coexistence of immunoreactivity to the peptides substance P, bombesin, calcitonin gene-related peptide and somatostatin has been determined in single, lumbar and sacral dorsal root ganglion cells in the cat. Colchicine pretreated L7 and S1 dorsal root ganglia were embedded in wax and cut into 5 μm sections. Groups of four, serially adjacent sections were reacted with antisera to one of four peptides using avidin-biotin immunocytochemistry. It was thus possible to determine the coincidence of the four peptides in single cell bodies by examining the immunoreactivity in a ganglion cell in one section and then locating the same cell in three adjacent sections. As a comparison, this procedure was repeated on a different population of ganglion cells using antiserum to substance P, bombesin and calcitonin gene-related peptide only. The results indicate that different combinations of three or four peptides may occur in single, small diameter sensory neurons in the cat. It would appear that immunoreactivity to bombesin and/or calcitonin gene-related peptide coexists with immunoreactivity to substance P in some dorsal root ganglion cells. However, immunoreactivity to each of these peptides was also found to occur alone in single cells. Immunoreactivity to calcitonin gene-related peptide but not to the other three peptides was found to occur in some medium-sized cell bodies (up to 70 μm). Somatostatin-like immunoreactivity was found to have a high level of coexistence with substance P-like immunoreactivity in cells which contained immunoreactivity to these two peptides only. Immunoreactivity to all the four peptides tested was found to occur in 18-26% of ganglion cells which contained at least one peptide.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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