Calculation of the molecular weight of proteins from electrophoretic and gel exclusion chromatographic experiments

Duggleby R.G. (1994) Calculation of the molecular weight of proteins from electrophoretic and gel exclusion chromatographic experiments. Bioinformatics, 10 2: 133-135. doi:10.1093/bioinformatics/10.2.133


Author Duggleby R.G.
Title Calculation of the molecular weight of proteins from electrophoretic and gel exclusion chromatographic experiments
Journal name Bioinformatics   Check publisher's open access policy
ISSN 1367-4803
Publication date 1994-04-02
Sub-type Article (original research)
DOI 10.1093/bioinformatics/10.2.133
Volume 10
Issue 2
Start page 133
End page 135
Total pages 3
Subject 1312 Molecular Biology
2613 Statistics and Probability
2605 Computational Mathematics
1706 Computer Science Applications
1703 Computational Theory and Mathematics
1303 Specialist Studies in Education
2701 Medicine (miscellaneous)
Abstract The molecular weights of proteins or protein subunits are frequently estimated from the electrophoretic mobility in the presence of sodium dodecyl sulphate, or from the elution volume in gel filtration chromatography. Using a series of standards of known size, a calibration curve is prepared which often yields a straight line in a semilogarithmic plot; interpolation from this line then gives the size of the sample. While this method is simple to apply, it does not give any estimate of the accuracy of the determined size. Furthermore there is no objective criterion for deciding if the calibration curve is linear, or a procedure for dealing with non-linear calibration curves. Here I report a method and a computer program which uses appropriate statistical methods to select the best straight or curved calibration line. The size of the unknown is then interpolated and an estimate or the error in this value is calculated.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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