Phenotypic analysis of B-cells extracted from human periodontal disease tissue.

Gemmell E. and Seymour G.J. (1991) Phenotypic analysis of B-cells extracted from human periodontal disease tissue.. Oral microbiology and immunology, 6 6: 356-362. doi:10.1111/j.1399-302X.1991.tb00507.x

Author Gemmell E.
Seymour G.J.
Title Phenotypic analysis of B-cells extracted from human periodontal disease tissue.
Journal name Oral microbiology and immunology   Check publisher's open access policy
ISSN 0902-0055
Publication date 1991-01-01
Sub-type Article (original research)
DOI 10.1111/j.1399-302X.1991.tb00507.x
Volume 6
Issue 6
Start page 356
End page 362
Total pages 7
Language eng
Subject 2403 Immunology
2726 Microbiology (medical)
3500 Dentistry
Abstract B‐cells extracted from periodontal disease tissue were analyzed for the presence of activation markers using a range of monoclonal antibodies. In adult periodontitis (AP), 6% of B‐cells expressed the IL‐2 receptor (CD25) compared with 1–2% in peripheral blood and healthy or marginal gingivitis (H/MG) gingival B‐cells. There was also an increase in the mean percentage of IgD‐positive B‐cells and a decrease in CD21 and CD22 expression. In both AP and H/MG lesions, 20–22% of the B‐cells expressed CD23 compared with < 5% in peripheral blood. As B‐cells are activated by day 3 in culture and start differentiating into immunoglobulin‐secreting cells by day 6, B‐cell phenotypes were assayed at these times in this study. Following stimulation with the periodontopathic bacterium Porphyromonas gingivalis, the expression of CD23, CD21 and CD22 on B‐cells extracted from AP lesions remained relatively constant over the 6‐d culture period. However, with Fusobacterium nucleatum stimulation, there was a significant decrease in CD23, CD21 and CD22 expression after 3 d in culture, which corresponds to the activation time for B‐cells. These results show that B‐cells extracted from periodontal disease tissue display a range of activation markers and on stimulation, demonstrate differing responses to individual periodontopathic bacteria. Copyright
Keyword B‐cell
periodontal disease
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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