Separation and characterization of endoglucanases from culture filtrates of Cellulomonas sp.

Prasertsan P. and Doelle H.W. (1986) Separation and characterization of endoglucanases from culture filtrates of Cellulomonas sp.. Applied Microbiology and Biotechnology, 24 4: 326-333. doi:10.1007/BF00257058


Author Prasertsan P.
Doelle H.W.
Title Separation and characterization of endoglucanases from culture filtrates of Cellulomonas sp.
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
Publication date 1986-01-01
Sub-type Article (original research)
DOI 10.1007/BF00257058
Volume 24
Issue 4
Start page 326
End page 333
Total pages 8
Publisher Springer-Verlag
Subject 2726 Microbiology (medical)
2404 Microbiology
1502 Banking, Finance and Investment
1305 Biotechnology
Abstract Six major components exhibiting endo-1,4-\-d-glucanase activity were partially purified from culture filtrates of a newly isolated Cellulomonas sp. using ion-exchange chromatography. Molecular weights (44,000 to 140,000), pH optima (6.0 to 7.0), temperature optima (40 to 50°C), half-life, energy of activation, Kmand other kinetic parameter investigations indicate the existence of 6 different endoglucanases. Further support for this assumption comes from inhibition studies, whereby glucose inhibited the enzyme activities between 15 and 50% at a concentration of 0.034% (1.65 mM) and cellobiose between 0 and 50% at a concentration of 0.1% (2.92 mM). Of all the metals (Hg2+, Co2+, Cu2+, Ca2+, Mg2+, Zn2+, Fe3+) tested, only Hg2+ exhibited a 55% inhibition at 5.0 mM.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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